Experiment of lymphocyte transformation with 3H-TdR——A study of method of sample preparation
|更新时间:2022-08-16
|
Experiment of lymphocyte transformation with 3H-TdR——A study of method of sample preparation
3H-TdR淋巴细胞转化试验中制样方法的探讨
Nuclear Techniques1980年03卷第6期
作者机构:
Jiangsu Institute of Nuclear Medicine, Wuxi
作者简介:
基金信息:
DOI:
中图分类号:
收稿日期:1979-03-12,
纸质出版日期:1980-12
稿件说明:
移动端阅览
Yang Xi-zhen, Wu Jia-ping, He Rong-xia, 等. Experiment of lymphocyte transformation with 3H-TdR——A study of method of sample preparation[J]. Nuclear Techniques, 1980,03(6):48-50.
Yang Xi-zhen, Wu Jia-ping, He Rong-xia, 等. Experiment of lymphocyte transformation with 3H-TdR——A study of method of sample preparation[J]. Nuclear Techniques, 1980,03(6):48-50.DOI:
During the experiment of lymphocyte transformation with
3
H-TdR
we found the counting rate of preparing sample using filter method was higher than that using chemical-digest method. Experiment showed the counting rate for 2
µ
Ci was doubled in filter method than digest method.
Moreover
when homemade 49 glass fiber disc was used in the filter method
the counting rate was markedly increased
in comparing with 0.8
μ
3μ
5μ millipore filter. The filtering rate was quite rapid and gave reproducible results. The liquid scintillator after filter disc being removed could be used over again.
关键词
Keywords
references
杨希震 , 江苏省原子医学研究所情况交流 , 1979 .
B. K. Schrier et al ., Methods Cell Biology , 13 , 105 ( 1976 ).