The preparation of tritium-labelled steroidsin high specific activity

Guo Zi-li; Tang Guo-zhong; Hua Ruo-ling

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The preparation of tritium-labelled steroidsin high specific activity

更新时间：2022-08-16

- The preparation of tritium-labelled steroidsin high specific activity
- 高比度氚标记甾族激素的制备
- Nuclear Techniques 1980年03卷第3期
- 作者机构：
  
  Shanghai Institute of Nuclear Research, Chinese Academy of Sciences
- 作者简介：
- 基金信息：
- DOI：
  中图分类号：
- 收稿日期：1978-12-18，
  
  纸质出版日期：1980-06
- 稿件说明：
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Guo Zi-li, Tang Guo-zhong, Hua Ruo-ling. The preparation of tritium-labelled steroidsin high specific activity[J]. Nuclear Techniques, 1980,03(3):50-53.

子丽郭, 国忠唐, 若苓华. 高比度氚标记甾族激素的制备[J]. 核技术, 1980, 03(3): 50-53.
Guo Zi-li, Tang Guo-zhong, Hua Ruo-ling. The preparation of tritium-labelled steroidsin high specific activity[J]. Nuclear Techniques, 1980,03(3):50-53. DOI：

子丽郭, 国忠唐, 若苓华. 高比度氚标记甾族激素的制备[J]. 核技术, 1980, 03(3): 50-53. DOI：

摘要

Abstract

在计划生育，内分泌学、针麻原理和临床医学研究中都需要测定体液中某些甾族激素的含量。使用高比度氚标记甾族化合物并运用竞争性蛋白质结合分析法（或放射免疫法）能使测量方法简便、用标本量少、结果可靠、灵敏度大大提高。此外，采用放射自显影技术研究甾族激素的代谢及生化作用时，氚标记甾族激素也是必不可少的示踪剂。

Seven tritiated steroids

H-testosterone

H-cortisol

H-corticosterone

H-progesterone

H-estradioletc

were prepared by the catalytical reduction of dehydrogenated precursor with tritium gas and were purified by thinlayer chromatography and paper chromatography. Products of 25Ci/m

to 85Ci/m

were obtained.t Their radiochemical purity was over 95%.

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Keywords

references

P. A. Osinski , Tritium in the Physical and Biological Sciences , Vol. 11 , IAEA , Vienna , 1962 , p 113 .

M. M. Coombs and H. R. Roderick , Nature , 203 , 523 ( 1964 ).

P. A. Osinski , Rec. Trav. Chim. , 79 , 216 ( 1960 ).

V. J. O'donnell and W. H. Pearlman , Biochem. J. , 69 , 38 ( 1958 ).

V. J. O'donnell et al ., Biochem. J. , 90 , 527 ( 1964 ).

M. Gut and M. Uskovic , J. Org. Chem. , 25 , 792 ( 1960 ).

S. K. Pradhan et al ., J. Org. Chem. , 29 , 601 ( 1964 ).

E. J. Agnello and G. D. Laulach , J. Amer. Chem. Soc. , 82 , 4293 ( 1960 ).

M. Akhtar et al ., J. Amer. Chem. Soc. , 85 , 1512 ( 1963 ).

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