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Shanghai Institute of Biochemistry, Chinese Academy of Sciences
收稿日期:1979-05-02,
纸质出版日期:1980-06
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Su Miao-gen. Radio-fluorography of tritium-labelled compounds[J]. Nuclear Techniques, 1980,03(3):27-30,35.
妙根 苏. 氚标记化合物的辐射荧光显影[J]. 核技术, 1980, 03(3): 27-30,35.
本文介绍硅胶薄层层析和聚酰胺薄膜层析方面的辐射荧光显影技术,它具有曝光时间短、分辩率和灵敏度高等优点 加入二甲苯闪烁液,在最佳温度-70℃下曝光,其灵敏度可比放射自显影提高100倍左右。
The technique of radio-fluorography used for silica-gel TLC and polyacrylamide TLC is described. It has the advantages of short time of exposure
high resolution and sensitivity. At the optimal temperature of - 70℃
with added xylene scintillator mixture
the sensitivity may be increased about 100 times as compared with the usual radioautography.
U. Luthi and P. G. Wasek , Nature , 205 , 1190 ( 1965 ).
S. Rotchild (Ed.), Advances in Tracer Methodology , Vol. 3 , Plenum Press , New york , 1966 , P. 149 .
K. Randerath , Anal. Biochem. , 34 , 188 ( 1970 ).
K. A. Laskey and A. P. Mills , Eur. J. Biochem. , 56 , 335 ( 1975 ).
W. Sawicki et al ., Histochem. , 52 , 341 ( 1977 ).
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