最新刊期
      卷 0 ， 期 0 ， 2020
      • LI Jinquan BAO Yaodong ZHOU Dai WU Yiwei JIANG Yimin
        Issue 11, (2001)
        摘要：To evaluate the cytocidal effect of 125 IUdR in human glioma cell line SHG44, 125 IUdR were added to the RPMI1640 culturing medium. The amount of 125 IUdR uptake by SHG44 cells was evaluated through measuring the radioactivity per cell; the killing effects of 125 IUdR on SHG44 cells were estimated by colony forming method. The amounts of 125 IUdR uptake by SHG44 were growing up as a function of 125 IUdR dose in the medium ( r =0.9917). The surviving fraction was correlated with the concentration of 125 IUdR ( r =-0.9736). The LD 50 of 125 IUdR group was (8.7±0.12)kBq/mL and the surviving fraction of 125 IUdR group was significantly lower than that of Na 125 I group ( P <0.001). 125 IUdR can be incorporated into the SHG44 cells, and the concentration of 125 IUdR in SHG44 cells was influenced by 125 IUdR dose in the medium. The inhibitive effects of 125 IUdR on SHG44 cells were obviously.  
        关键词：Cerebral glioma;125 IUdR;SHG44 cells;Radioisotope-therapy   
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        发布时间：2021-03-12
      • FENG Huiru TIAN Jiahe ZHANG Jingming
        Issue 11, (2001)
        摘要：In an attempt to understand radiobiological basis for targeted radiotherapy in oncology, the cell cycle perturbations have studied in Hela cell lines after exposed to different doses and dose-rate of 32 P radiation. Asynchronous Hela cells, cultured in vitro, were exposed to β radiation from radioactive filter papers (absorbed 32 P) which were put close under culture plate of growing monolayer of Hela cells. The characteristic radiation response to different dose, dose-rate and radiation time was evaluated through cell cycle perturbation studied by flow cytometry. Cell cycle status showed G2 phase blockage in a way of dose dependence, a plateau of G2 block can be recognized at about 24h. Interestingly, the G2 phase declined even though the accumulated doses increased as the time of radiation prolonged. This result suggested that the cell cycle progress could not be inhibited completely when exposed to continuous radiation, rather it seems to be controlled somehow by the nature of cell cycle itself for a certain cell line. G2 blockage, one of the major changes caused by β radiation, is dose-dependent, but the time reaching the plateau of G2 phase blockage is most likely related with the intrinsic nature of cell cycle.  
        关键词：Targeted radiotherapy;Radiobiological mechanism;Cell line;Cell cycle   
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        发布时间：2021-03-12
      • XIAO Hualong 1 HUANG Biao 2 ZHU Liguo 1 TAN Cheng 2 CAI Gangming 2 JIN Jian 2 1
        Issue 11, (2001)
        摘要：To establish an IFMA of anti-HCV, the antigen of HCV was covered on the microwell plate, and then incubated with human anti-HCV in sample and Eu 3+ labeled goat anti-human IgG. The luminescent enhancement solution contains mainly 2-napthoyltrifluororacetate, and the data was treated with the log-logit function and four- parameter logistic function. Results showed that the intra- and inter- assay CV of the IFMA was 3.64% and 4.39%, respectively, and the recovery rate was 105.58%, the sensitivity was 0.03NCU/mL. The cross reactivity with HBsAg and HbeAg was 0.23% and 0.04%, respectively. The anti-HCV concentrations in serum of 278 health persons were all less than 1NCU/mL. Results indicated this new method was sensitive, stable and could be used as an effective non-radioassay tool for the basic or clinical study on HCV.  
        关键词：Anti-HCV;Time-resolved fluorimmunoassay   
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        发布时间：2021-03-12
      • WANG Jufang HE Jing ZHOU Guangming LI Wenjian WEI Zengquan
        Issue 11, (2001)
        摘要：In this paper, DNA strand breakage induced by γ-ray was studied using the Comet Assay. The human hepatoma cells were chosen as the test cell line. The results showed that the length of the tail appeared longer as the dose increased, and the number of cells with tail, increased with increasing dose. The length ratio and area ratio had positive correlation with the dose. Among them, the length ratio showed more obvious change with the increasing dose than area ratio, thus it could be used to describe the level of DNA strand breakage simply and efficiently.  
        关键词：Comet Assay;γ-ray;Human hepatoma cell;DNA strand breakage   
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        发布时间：2021-03-12
      • ZUO Chuantao LIU Yongchang GUAN Yihui ZHAO Jun LIN Xiangtong
        Issue 11, (2001)
        摘要：To evaluate the role of 18 FDG-PET in the differential diagnosis of recurrence and radionecrosis in glioma. FDG-PET scans in 15 glioma patients were performed after initial surgery and radiation therapy. Results of the 12 tumor recurrence, 11 FDG-PET scans showed hypermetabolism, 3 patients with hypometabolic abnormalities on FDG-PET scan had no evidence of recurrence. In recurrence, the primary site showed platelike, ringform and tocal FDG uptake and also increased uptake in remote area. In tumor recurrent patients, the lesion’s highest radioactivity/average white matter radioactivity ratio was 2.85±1.0, while the ratio of radionecrosis was 1.32±0.07, the differences were significant. Conclusions show that FDG-PET scans are helpful in the differentiation between tumor recurrences and radionecrosis in glioma.  
        关键词：FDG-PET;Glioma;Recurrence;Radionecrosis   
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        发布时间：2021-03-12
      • WANG Changyin HONG Shufang CHENG Xiaoli WEN Bing ZHANG Xiangyuan
        Issue 11, (2001)
        摘要：normal subjects and 250 patients with thyroid disease were performed thyroid imaging. The parameters reflecting the function of thyroid were calculated,including rectified ratio(RR),uptake ratio(UR) and 99m Tc percentage uptake(PU).The identity between RR and PU was compared with the identify between UR and PU. The parameters in disease groups were also compared with that of normal group. The results showed that the real identify percentage (95.6%) between RR and PU was significantly higher than that of UR and PU (77.9%) ( χ 2=40.9, P <0.001). RR were significantly higher in groups of hyperthyroidism and recurrence after thyroidectomy than normal group ( P <0.05-0.005),and significantly lower in subacute thyroiditis group ( P <0.001).RR may be normal or increased in simple goiter and may be normal, increased or decreased in hypothyroidism. Thereby, RR is obviously better than UR in determining the level of thyroid 99m Tc uptake. It is difficult in discriminating between hyperthyroidism and hypothyroidism by RR, but it still has important significance in correlation with clinical and other examination data for comprehansive judgement.  
        关键词：Thyroid;99m Tc;Ratio of thyroid with neck background   
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        发布时间：2021-03-12
      • DU Tongxin WANG Zizheng SUN Junjiang WANG Shukui QI Shaokang
        Issue 11, (2001)
        摘要：To deplore the relationship between leptin and c-peptide in children with type 1 diabetic mellitus (DM). The levels of serum leptin and c-peptide(C-P) in 65 type 1 DM children (including 31 before and after insulin treatment) and 30 normal controls were measured by radioimmunoassay (RIA). The results found that there was significant differences ( P <0.01) in leptin and C-P between DM children and normal controls, also in 31 DM children before and after treatment. It showed a positive correlation between leptin and C-P. The changes of the leptin/C-P ratio in DM children compared with normal controls and that before and after treatment were also significantly different. It suggested that leptin may have close relationship in the development, progress and the occurrence of complications in children with DM and also provide a new clue for their diagnosis treatment and complication occurrence.  
        关键词：Children type 1 diabetic mellitus;Leptin;C-peptide;Radioimmunoassay   
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        发布时间：2021-03-12
      • SU Chenghai BI Peiying JIANG Yimin WU Yiwei ZHANG Wei DONG Shen’an SANG Shibiao CHEN Weichang XU Fengpo WU Xinghong
        Issue 11, (2001)
        摘要：The heart/liver ratio (H/L) and portal vein pressure (PVP) in 54 patients of cirrhosis with various liver function were determined by the rectal administration of 99m Tc-MIBI, The H/L ratio in Child A (21 cases), Child B (15 cases) and Child C (18 cases) group was 0.49±0.18, 0.69±16 and 0.91±0.21 respectively, whereas the PVP was 2.22±0.57kPa, 2.85±0.38kPa and 3.54±0.65kPa respectively. In normal controls (16 cases), the H/L ratio and PVP was 0.30±0.12 and 1.61±0.38kPa. There was no significant difference in PVP between Child B and C groups, but has very significant ( P <0.001) and significant ( P <0.05) difference of H/L ratio and PVP in remaining groups after t test. In 16 cirrhotic patients the H/L ratio and PVP was 0.73±0.16 and 3.00±0.55 before treatment, and become 0.47±0.18 and 2.20±0.62kPa after treatment, there was also very significant difference after t-test. It was concluded that this method for the determination of PVP is not only convenient, but also atraumatic and acceptable for the patient. Thereby, it has unique value for the staging, monitoring therapeutic effect, guiding treatment protocol and jndging the prognosis etc. in cirrhotic patients.  
        关键词：m Tc-MIBI;Reagent put into rectum;Cirrhosis;Portal vein pressure;Estimating curative effect   
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        发布时间：2021-03-12
      • DONG Mo LI Huiyuan WU Yuanfang
        Issue 11, (2001)
        摘要：Human peripheral blood exposed to 188 ReO - 4 at various doses for 54h was examined to observe the chromosome and chromatin aberrations at metaphases during mitosis of lymphocytes. The findings indicate that there was a tendency of increased aberration in both types with increasing 188 ReO - 4 concentration and a dose dependency can be seen. The aberration yield is best fitted by the quadratic model. Chromatin aberrations, aberrations cell and chromosome fragments are also fitted by a linear-regression model.  
        关键词：ReO - 4;Peripheral blood;Chromosome aberration   
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        发布时间：2021-03-12
      • WANG Mingfang 1 TANG Ganghua 1 GAO Xiao 1 LI Zhi 1 CHEN Yuyao 2 WU Hubing 1 HUANG Zuhai 1 1
        Issue 11, (2001)
        摘要：To study the synthetic method, quality control and biodistribution of 13 N-ammonia, 13 N-NO - 3 and 13 N-NO - 2 were produced from water via the 16 O(p,α) 13 N nuclear reaction with 16.5 MeV proton at 25-30 μA for 10-20 min and then converted to 13 N-ammonia by the Devarda’s alloy. Radiochemical purity was better than 95% as determined by HPLC and TLC. The biodistribution study of 13 N-ammonia in mice indicated that the mean uptake of 13 N-ammonia in myocardium was as high as 19.54%. At the first-pass time, the uptakes of myocardial and lung were (24.90±1.82)% and (22.79±1.94)%, respectiviely, and 92% radioactivity was cleared from blood. After injection, the radioactivity in each organ was significantly readuced at 1 min and was minimum at 5min, but significantly increased at 10min. The uptake of myocardium varied within the range of 11.34-29.24 during 1-30min. High heart-to-liver and heart-to-lung ratios and rapid blood clearance were found. The results suggust that the Devarda’s alloy method is a simple and rapid reducing means for the preparation of 13 N-ammonia. 13 N-ammonia was suitable for clinical regional blood perfusion imaging. PET imaging is suitable at 2-5min after injection.  
        关键词：Preparation of 13 N-ammonia;Positron imaging agent;Quality control;Biodistribution   
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        发布时间：2021-03-12
      • LU Jie LU Gongxu WANG Xuebing
        Issue 11, (2001)
        摘要：On the basis of successfully obtained [ 99m TcN] 2+ int core, the complex 99m TcN-tetrofosmin was prepared through the ligand-exchange reaction. The radiochemical purity of 99m TcN-tetrofosmin was over 95% by TLC. A study of stability indicated the complex was stable over 6h at room temperature. The mice biodistribution of 99m TcN-tetrofosmin showed the accumulation in myocardium with high retention and rapid blood and lung clearance.  
        关键词：Myocardial imaging agent;Tetrofosmin;99m TcN-tetrofosmin;Biodistribution   
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        发布时间：2021-03-12
      • YU Junfeng YIN Duanzhi CUI Haiyan XU Jianying ZHANG Lei ZHOU Wei MIN Xiaofeng CHEN Minfeng TAN Mingguang WANG Yongxian
        Issue 11, (2001)
        摘要：The 188 Re sulfide suspension could be prepared quickly by a kit. The kit consists of three 10mL vials. All the procedures were carried out in sterility circumstance. Vial A consisted of 1mL KReO 4 and Na 2S 2O 3 (mole ratio 1:70) solution with excipient. Vial B was 5mol/L HCl solution. Vial C consisted of 1.5mL PVP and NaOH solution (mole ratio 1000:1). Vial B was sealed, Vial A and C were freeze-dried. Bacterium check, endotoxin check and security check were qualified. Kits were kept at ambieut temperature and the Labelling efficiency of 188 Re sulfide suspension was more than 98% with 2 months. The particle sizes were 1-5μm (60.1±12.7)%; 5-10μm(30.9±8.1)%; >10μm(9.0±4.7)%. The radiochemical purity of 188 Re sulfide suspension made by the kit was higher than 98% in 5 days with particle sizes 1-5μm(49.9±4.4%); 5-10μm(41.1±5.4)%; >10μm(9.0±3.3)%.  
        关键词：Re sulfide;Suspension;Kit   
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        发布时间：2021-03-12
      • ZHANG Junbo WANG Xuebin
        Issue 11, (2001)
        摘要：The aim of this study is to examine the influence on biodistribution by introducing the [ 99m Tc≡N] 2+ core into the complex so as to explore a new radiopharmaceutical. 99m TcN-IBDTC (IBDTC:N-isobutyl dithiocarbamate) was prepared through a two-step ligand-exchange reaction by using SnCl 2·2H 2O as reduction agent and SDH (succinic dihydrazide) as a donor of nitride nitrogen atom (N 3- ) at room temperature. The ligand IBDTC was labelled with 99m TcO - 4 directly by using FSA (Formamidine sulfinic acid) as reducing agent. The radiochemical purity of the 99m TcN-IBDTC and 99m Tc-IBDTC complex were both over 90% by TLC. The biodistribution results in mice indicated that 99m TcN-IBDTC had high brain uptake and good brain retention. The brain uptake (%ID·g -1 ) was 6.22, 5.45 and 3.88 and the brain/blood ratios were 1.51, 2.24 and 1.84 at 5, 30 and 60min post-injection respectively. The results suggested that it would be potentially useful as a brain perfusion imaging agent. 99m TcN-IBDTC also exhibited high myocardial uptake, but the washout from the heart was rapid and the heart/liver ratio was low thereby restricting the use of the complex as a myocardial perfusion imaging agent. The biodistribution results in mice suggested 99m Tc-IBDTC was mostly accumulated in liver, whereas the brain and heart uptake was very low. This study proves that the introduction of the [ 99m Tc≡N] 2+ core into the molecular structure of a radiopharmaceutical may obviously alter its biological behavior. This may be valuable for the design of new radiopharmaceuticals for clinical use.  
        关键词：m TcN-IBDTC;99m Tc-IBDTC;Radiopharmaceutical;Heart and brain uptake;Biodistribution   
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        发布时间：2021-03-12
      • ZHANG Jinming 1 TIAN Jiahe 1 CAO Limin 1 BAI Hongsheng 2 1
        Issue 11, (2001)
        摘要：Y-EDTA was eluted from 90 Sr/ 90 Y generator, labeled activation charcoal. The upper liquid was removed with centrifugation. The labeled yield was over 95%. 90 Y-charcoal was very stable in saline. After i.m or i.p to mice, the over 99% radioactivity were remained in the point of injection after 72h. The uptake of femur is (0.0019±0.0010)%ID/g by i.m, and (0.0453±0.0051)%ID/g after 72h by i.p. The other’s organic activity is nearly background. In conclusion, 90 Y-charcoal is stable and doesn’t degraded in vivo. It suggested that 90 Y-charcoal may be a potential agent for internally therapeutic use as other particles like glass-microsphere.  
        关键词：Charcoal;Radionuclide therapeutic;Yttrium-90   
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        发布时间：2021-03-12
      • FENG Dinghua 1) ZHANG Zeng 2) FU Yindi 3) MO Yahong 1) CHENG Qijun 1) 1
        Issue 11, (2001)
        摘要：The dose profiles at the center and the places 1cm away from the surface of a standard dosimetry phantom of a head were measured with thermoluminescent dosimeters (TLD) by using a Hitachi W-1000 CT system. Regression equations for the scattered radiation dose as a function of the distance from the radiation center were obtained by fitting the measured data. The CT dose index (CTDI) was different from those computed by integrating over different intervals. Some problems worthy of special attention were discussed. This study provides a technical base for CT dose monitoring and promoting application of International Basic Standard of Radiation Protection and The Safety of Radiation Source (IBSS) guide level.  
        关键词：Head dosimetry phantom;TLD;Computed tomography dose index;Multiple scan average dose   
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        发布时间：2021-03-12
      • ZHANG Xiaodong LI Wenxin
        Issue 11, (2001)
        摘要：Radioactive stents is a promising avenue to reduce the rate of restenosis after percutaneous transluminal coronary angioplasty (PTCA). In this paper, the preparation methods of radioactive stents including ion implantation, activation in reactor or in cyclotron and surface handling methods were reviewed. Ion implantation was used to produce the 32 P-implanted stent undergoing clinic evaluation. Other methods also showed their respective advantages. The composit polymer stent or nitinol stent may be the next aims to be explored in this field.  
        关键词：PTCA;Radioactive stent;Ion implantation;Activation;Surface handling   
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        发布时间：2021-03-12
      • ZHANG Chunfu ZHANG Xiuli ZHANG Yongping WANG Yongxian
        Issue 11, (2001)
        摘要：This paper reviewed the method and principle of brachytherapy, the characteristics of main radionuclides, the development and current situation of the techniques, and the criteria for patient selection. The types of seed distribution, dose selection and current problems were also discussed.  
        关键词：Brachytherapy;Prostate cancer;103 Pd;125 I   
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        发布时间：2021-03-12
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