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Protective effect of phycocyanin against oxidative damage induced by radiation
- NUCLEAR TECHNIQUES Vol. 41, Issue 1, Article number: 010302(2018)
- 作者机构：
1.烟台大学 药学院 烟台 264005
2.中国科学院烟台海岸带研究所 烟台 264003
3.兰州大学 核科学与技术学院 兰州 730000
- 作者简介：
- 基金信息：Strategic Priority Research Program of Chinese Academy of Sciences;Ocean Public Welfare Scientific Research Project, State Oceanic Administration of China;National Natural Science Foundation of China;National Natural Science Foundation of China;National Key Technology R & D Program of China;Science and Technology Program of Yantai;Regional Demonstration of Marine Economy Innovative Development Project
DOI：10.11889/j.0253-3219.2018.hjs.41.010302
CLC： TL7;R963Received：28 June 2017，
Revised：07 September 2017，
Published：10 January 2018
- 稿件说明：
移动端阅览
Qi LIU, Wenjun LI, Lina LU, et al. Protective effect of phycocyanin against oxidative damage induced by radiation[J]. Nuclear techniques, 2018, 41(1): 010302
Qi LIU, Wenjun LI, Lina LU, et al. Protective effect of phycocyanin against oxidative damage induced by radiation[J]. Nuclear techniques, 2018, 41(1): 010302 DOI： 10.11889/j.0253-3219.2018.hjs.41.010302.
摘要
通过建立X射线辐射损伤小鼠模型，研究藻蓝蛋白（Phycocyanin，PC）对辐射引起鼠氧化损伤的保护作用。选取C57 BL/6雄性小鼠72只，按体重随机分为正常对照组、单纯辐射组、PC预防组、阳性对照组，每组18只。辐射前连续灌胃相应受试样品7 d，在第8 d，除正常组外，其余各组接受全身一次性6 Gy的X射线照射。各组分别在辐射后的1 d、3 d、7 d留取血浆和组织，并检测血浆及肝组织中的抗氧化酶活性及肝组织中活性氧自由基（Reactive Oxygen Species，ROS）含量。实验结果表明，藻蓝蛋白显著提高了辐射后小鼠血浆中和肝脏中抗氧化酶超氧化物歧化酶（Superoxide Dismutase，SOD）、谷胱甘肽过氧化物酶（Glutathione Peroxidase，GSH-PX）的活性（
p
<
0.05），降低了丙二醛（Malondialdehyde，MDA）的含量（
p
<
0.05），减少肝组织中ROS的含量（
p
<
0.05）。结果表明，藻蓝蛋白可提高小鼠抗氧化能力，减轻辐射对机体造成的氧化损伤，对小鼠辐射损伤有较好的防护作用。
Abstract
Background
2
Phycocyanin (PC) is a pigment protein present in cyanobacteria
cryptophyta
etc
.
Purpose
2
This study aims at the protective effect of phycocyanin on oxidative damage induced by irradiation by establishing injury mouse model by X-ray irradiation.
Methods
2
72 C57 BL/6 male mice were randomly divided into normal control group
irradiation model group
PC pretreatment group and positive control group. The mice were continuously fed for 7 d. On the 8th day of intragastric administration
except for the normal control group
the rest of the mice were subjected to total body irradiation to a dose of 6 Gy for the preparation of radiation damage model. The plasma and tissues were collected at 1 d
3 d and 7 d after irradiation
and the activities of antioxidant enzymes in plasma and liver were measured. The content of reactive oxygen species (ROS) in liver tissue was detected by dihydroethidium (DHE) staining.
Results
2
Phycocyanin increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) in the plasma of mice after irradiation
and increased the activity of SOD (
p
<
0.05)
decreased malondialdehyde (MDA) content (
p
<
0.05)
and reduced liver tissue ROS content (
p
<
0.05).
Conclusion
2
The results show that phycocyanin can improve the antioxidant capacity of mice
reduce oxidative damage to the body caused by irradiation
and provide a better protective effect on irradiation injury of mice.
关键词
Keywords
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