Catalytic polymerization-based X-ray imaging tags

Ying ZHU; Huating KONG; Jichao ZHANG; Lihua WANG; Jun HU; Chunhai FAN

doi:10.11889/j.0253-3219.2017.hjs.40.060103

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Catalytic polymerization-based X-ray imaging tags

SYNCHROTRON RADIATION TECHNOLOGY AND APPLICATIONS | 更新时间：2021-05-19

- Catalytic polymerization-based X-ray imaging tags
- NUCLEAR TECHNIQUES Vol. 40, Issue 6, Article number: 060103(2017)
- 作者机构：
  
  中国科学院上海应用物理研究所物理生物学研究室中国科学院微观界面物理与探测重点实验室上海同步辐射光源生物成像中心嘉定园区上海 201800
- 作者简介：
- 基金信息：
  
  Open Large Infrastructure Research of Chinese Academy of Sciences;National Key Research and Development Program;National Natural Science Foundation of China;National Natural Science Foundation of China;the Youth Innovation Promotion Association of Chinese Academy of Sciences
- DOI：10.11889/j.0253-3219.2017.hjs.40.060103
  CLC： TL99;O63
- Received：13 April 2017，
  
  Revised：21 April 2017，
  
  Published：10 June 2017
- 稿件说明：
移动端阅览
Ying ZHU, Huating KONG, Jichao ZHANG, et al. Catalytic polymerization-based X-ray imaging tags[J]. Nuclear techniques, 2017, 40(6): 060103
DOI：

Ying ZHU, Huating KONG, Jichao ZHANG, et al. Catalytic polymerization-based X-ray imaging tags[J]. Nuclear techniques, 2017, 40(6): 060103 DOI： 10.11889/j.0253-3219.2017.hjs.40.060103.

摘要

同步辐射X射线显微成像具有极高的空间分辨率、很好的穿透深度和优秀的能量分辨，因而在纳米分辨细胞成像领域具有巨大的应用潜力。然而，目前X射线显微技术多用于细胞结构成像，而和该技术相适合的特异性识别细胞内重要生物靶标的分子探针仍较缺乏。本工作利用同步辐射X射线良好的能量分辨特点，经体外化学催化反应成功制备了同步X射线可见的成像标签，成像分辨率达到30 nm。研究结果为进一步制备X射线敏感的分子探针，实现对细胞内生物分子的特异性识别和成像打下了良好的基础。

Abstract

Background

With short wavelengths

long penetration depth and excellent energy resolution of X-rays

synchrotron-based X-ray microscopy (XRM) has demonstrated its great potential for nanoscale cellular imaging. However

despite of the high-quality sub-cellular morphological details provided by XRM

it lacks appropriate molecular probes capable of localizing cellular targets.

Purpose

This study aims to perform the preparation of X-ray imaging tags.

Methods

In vitro

chemical catalytic reaction was introduced to get the X-ray imaging tags.

Results

By exploiting the excellent energy resolution of X-rays

synchrotron-based X-ray imaging tags were prepared with the imaging resolution of 30 nm when observed by soft X-ray microscopy at Shanghai synchrotron radiation facility (SSRF).

Conclusion

The results indicate high potential to design X-ray sensitive molecular probes for cell imaging.

关键词

Keywords

references

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