An automatic solution-sample-changing peristaltic device at biological small angle X-ray scattering beamline

Chunxia HONG; Ping ZHOU; Yiwen LI; Jianrong ZENG; Fenggang BIAN; Jie WANG

doi:10.11889/j.0253-3219.2016.hjs.39.010102

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An automatic solution-sample-changing peristaltic device at biological small angle X-ray scattering beamline

更新时间：2021-05-24

- An automatic solution-sample-changing peristaltic device at biological small angle X-ray scattering beamline
- NUCLEAR TECHNIQUES Vol. 39, Issue 1, Article number: 010102(2016)
- 作者机构：
  
  1.中国科学院上海应用物理研究所张江园区上海 201204;
  2.中国科学院大学北京 100049
- 作者简介：
- 基金信息：
- DOI：10.11889/j.0253-3219.2016.hjs.39.010102
  CLC： TP273+.5
- Received：26 October 2015，
  
  Accepted：2015-12-29，
  
  Published：25 January 2016
- 稿件说明：
移动端阅览
Chunxia HONG, Ping ZHOU, Yiwen LI, et al. An automatic solution-sample-changing peristaltic device at biological small angle X-ray scattering beamline[J]. Nuclear techniques, 2016, 39(1): 010102
DOI：

Chunxia HONG, Ping ZHOU, Yiwen LI, et al. An automatic solution-sample-changing peristaltic device at biological small angle X-ray scattering beamline[J]. Nuclear techniques, 2016, 39(1): 010102 DOI： 10.11889/j.0253-3219.2016.hjs.39.010102.

摘要

利用同步辐射X射线小角散射技术可以研究溶液中蛋白质的结构信息。但是在实验过程中

高通量的X射线易造成蛋白质的辐射损伤

发生结构变化。本文介绍了一种自动换样溶液样品蠕动装置

在实验过程中利用Hamilton的PSD/4注射泵控制样品上下运动

减小单位体积照射时间以降低X射线对蛋白质的辐射损伤。此外

通过对注射泵、准直调节台和样品/缓冲液支撑台的协调控制实现了自动换样、回样和清洗功能

提高了实验效率。在上海光源生物X射线小角散射实验站进行了实验

通过对静止模式和蠕动模式下溶菌酶的散射曲线及回旋半径的测量

表明该装置可达到很好的防辐射损伤效果

实现了预期的样品蠕动装置功能。

Abstract

Background:

Protein structure in solution can be studied by small-angle X-ray scattering techniques at synchrotron radiation facilities. However

the structure of protein is prone to vary as the radiation damage induced by the intense X-ray beam during the experiments.

Purpose:

This study aims to develop an automatic solution-sample- changing peristaltic device for biological small angle X-ray scattering experiment.

Methods:

The injection pump PSD/4 of Hamilton is applied to eliminate the radiation damage of protein by minimizing the X-ray irradiation time of unit volume. In addition

automatic sample-changing

retrieving and cleaning was realized by the coordinated control of PSD/4

the alignment control stage and the sample/buffer control stage. Thus the experiment efficiency is improved.

Results:

The scattering curves and gyration radius of lysozyme under stationary collection mode and peristaltic collection mode were measured by experiments carried out at biological small angle X-ray scattering beamline of Shanghai Synchrotron Radiation Facility (SSRF). And results show that this device can prevent radiation damage of sample efficiently.

Conclusion:

The designed functionalities have been achieved as expected.

关键词

Keywords

references

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