Electron microscopic and agarose gel electrophoretic studies on apoptosis in immune cells induced by enriched <sup>235</sup>U

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Electron microscopic and agarose gel electrophoretic studies on apoptosis in immune cells induced by enriched ²³⁵U

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- Electron microscopic and agarose gel electrophoretic studies on apoptosis in immune cells induced by enriched ²³⁵U
- NUCLEAR TECHNIQUES Issue 5, (1998)
- 作者机构：
  
  苏州医学院,苏州,215007
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392.1
- Published：01 May 1998
- 稿件说明：
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[1]朱寿彭,张澜生,付强.浓缩~(235)U诱发免疫细胞凋亡的电镜和琼脂糖凝胶电泳研究[J].核技术,1998(05):284-288.

Electron microscopic and agarose gel electrophoretic studies on apoptosis in immune cells induced by enriched ²³⁵U[J]. Nuclear techniques, 1998, (5): 284-288.
[1]朱寿彭,张澜生,付强.浓缩~(235)U诱发免疫细胞凋亡的电镜和琼脂糖凝胶电泳研究[J].核技术,1998(05):284-288. DOI：

Electron microscopic and agarose gel electrophoretic studies on apoptosis in immune cells induced by enriched ²³⁵U[J]. Nuclear techniques, 1998, (5): 284-288. DOI：

摘要

研究了浓缩235U内照射诱发人淋巴细胞白血病细胞株Molt－4细胞和巨噬细胞株Ana－1细胞的细胞调亡。估算了浓缩235U在不同阶段培养细胞中的辐射累积吸收剂量。通过电镜对免疫细胞的形态观察，发现Molt－4和Ana－1细胞在受235U内照射作用下可呈现核染质边聚，DNA链断裂和膜包裹的凋亡小体形成。对Molt－4和Ana－1细胞的DNA抽提，进行DNA琼脂糖凝胶电泳也显示出阶梯状条带形成的细胞调亡特征。从而证实了浓缩235U内照射可导致免疫细胞Molt－4和Ana－1细胞的凋亡发生。

Abstract

At present

the apoptosis in Molt-4 cell（a humaan acute lymphoblastic leukemiacell line） and Ana-1 cell （a macrophage cell line） were studied after internalirradiation with enriched 235U. The cumulative radiation absorption dose of 235U incultural cells through different periods were estimated

The morphological changes

which observed by electron microscope

indicated that Molt-4 and Ana-1 immunecells after incubation with 235U

displayed nuclear fragmentation

margination ofcondensed chromatin

as well as the membrane-bounded apoptotic bodies formation.The agarose gel electrophoretic observations showed the DNA ladder paternformation in Molt-4 cell as well as in Ana-1 cell. The experimental results showedthat apoptosis induced by 235U in immune cells

were dependent on the 235U-treatedtime and cumulative radiation absorption dose.

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Electron microscopic and agarose gel electrophoretic studies on apoptosis in immune cells induced by enriched 235U

DOI：

摘要

Abstract

关键词

Keywords

references

Electron microscopic and agarose gel electrophoretic studies on apoptosis in immune cells induced by enriched ²³⁵U