Preparation of Re labelled antibodies

ZHU Minghua CAO Rongzhen LI Wenxin SHENG RongYIN Duanzhi HE Weiyu ZHOU Wei WANG Yongxian

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Preparation of Re labelled antibodies

更新时间：2021-04-09

- Preparation of Re labelled antibodies
- NUCLEAR TECHNIQUES Issue 7, (1998)
- 作者机构：
  
  中国科学院上海原子核研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： TL923
- Published：01 July 1998
- 稿件说明：
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[1]朱明花,曹蓉珍,李文新,盛荣,何蔚瑜,周伟,汪勇先.抗-CEA单抗的188Re标记[J].核技术,1998(07):411-414.

ZHU Minghua CAO Rongzhen LI Wenxin SHENG RongYIN Duanzhi HE Weiyu ZHOU Wei WANG Yongxian. Preparation of Re labelled antibodies[J]. Nuclear techniques, 1998, (7).
[1]朱明花,曹蓉珍,李文新,盛荣,何蔚瑜,周伟,汪勇先.抗-CEA单抗的188Re标记[J].核技术,1998(07):411-414. DOI：

ZHU Minghua CAO Rongzhen LI Wenxin SHENG RongYIN Duanzhi HE Weiyu ZHOU Wei WANG Yongxian. Preparation of Re labelled antibodies[J]. Nuclear techniques, 1998, (7). DOI：

摘要

采用转移能力更强的葡萄糖酸钠和ＳｎＣｌ２的混合液及Ｎａ１８８ＲｅＯ４，用直接标记法标记了抗－ＣＥＡ单抗。研究了影响标记率的若干因素，如抗体和抗坏血酸的用量、亚锡的浓度、反应液ｐＨ值以及反应时间等，探索出标记率可达８０％以上的标记条件。

Abstract

A simple technique of directly labelling antibodies with Re has been developed.The reduction of antibody disulfide groups was achieved by incubation of antibodywith ascorbic acid （pH=6.5）for an hour at room temperature and a solution of excessSnCl2 in sodium gluconate was added to the AA-reduced antibody followed by theaddition of perrhenate

Some factors that influence labelling efficiency

such as thepH of the reaction mixture

the labelling time

and the amount of antibodies andreductive agent

were studied experimentally and a better labelling method wasestablished

The labelling yidlds

as determined by paper chromatography

weregreater that 80%.

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