Preparation of tritium labelled thymidine with high specific activity

Dong Mo   Cao Rongzhen

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Preparation of tritium labelled thymidine with high specific activity

更新时间：2021-04-09

- Preparation of tritium labelled thymidine with high specific activity
- NUCLEAR TECHNIQUES Issue 1, (1997)
- 作者机构：
  
  中国科学院上海原子核研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： TL93
- Published：01 January 1997
- 稿件说明：
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[1]董墨,曹蓉珍.高比活度[甲基一~3H]胸腺嘧啶核苷的制备[J].核技术,1997(01):50-53.

Dong Mo Cao Rongzhen. Preparation of tritium labelled thymidine with high specific activity[J]. Nuclear techniques, 1997, (1).
[1]董墨,曹蓉珍.高比活度[甲基一~3H]胸腺嘧啶核苷的制备[J].核技术,1997(01):50-53. DOI：

Dong Mo Cao Rongzhen. Preparation of tritium labelled thymidine with high specific activity[J]. Nuclear techniques, 1997, (1). DOI：

摘要

以５—甲酰基尿嘧啶为起始原料，采用“二步法”：第一步合成了氚标记的５—羟甲基尿嘧啶，第二步合成了氚标记的胸腺嘧啶。放射性比活度分别达到１．６ＰＢｑ／ｍｏｌ和２．２ＰＢｑ／ｍｏｌ，再经酶促反应，生成氚标记胸腺嘧啶核苷，其比活度达２．２ＰＢｑ／ｍｏｌ，放化纯度大于９５％

Abstract

Methy1 3H] thymine was synthesized from 5 formyluracil by the “two-stepmethod”.As the first step

5 [hydroxymethy1 3H] hydroxymethyluracil was prepared with specific radioactivity of 1.6PBq/mol.Then [methy1 3H]thymine was synthesizedvia enzymatic reaction with specific radioactivity of 2.2PBq/mol.The radiochemical purity of the product was over 95%.

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曹蓉珍

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Related Institution

Shanghai Institute of Nuclear Research, Academia Sinica

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Shanghai Institute of Nuclear Research, Academia Sinica, shanghai 201800)Lan Qing

Institute of Atomic Energy of China

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