Preparation of         125  I-Tumor necrosis factor mutants by iodogen and the measurement of its specific radioactivity

Ran Ruiqiong    1     Wang Guoping    2     Tang Guozhong    2    Wu Yuxin    3     Zhu Chengmo    3     Cao Shilong    1    1

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Preparation of 125 I-Tumor necrosis factor mutants by iodogen and the measurement of its specific radioactivity

更新时间：2021-04-09

- Preparation of 125 I-Tumor necrosis factor mutants by iodogen and the measurement of its specific radioactivity
- NUCLEAR TECHNIQUES Issue 5, (1997)
- 作者机构：
  
  1. 上海医科大学附属肿瘤医院
  2. 中国科学院上海原子核研究所
  3. 上海第二医科大学附属瑞金医院
  4. 上海医科大学附属肿瘤医院中国科学院上海原子核研究所上海第二医科大学附属瑞金医院
- 作者简介：
- 基金信息：
- DOI：
  CLC： R730.23
- Published：01 May 1997
- 稿件说明：
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[1]冉瑞琼,王国平,唐国忠,朱承谟,曹世龙.肿瘤坏死因子突变体的碘标记及其质量的检测[J].核技术,1997(05):29-32.

Ran Ruiqiong 1 Wang Guoping 2 Tang Guozhong 2 Wu Yuxin 3 Zhu Chengmo 3 Cao Shilong 1 1. Preparation of 125 I-Tumor necrosis factor mutants by iodogen and the measurement of its specific radioactivity[J]. Nuclear techniques, 1997, (5).
[1]冉瑞琼,王国平,唐国忠,朱承谟,曹世龙.肿瘤坏死因子突变体的碘标记及其质量的检测[J].核技术,1997(05):29-32. DOI：

Ran Ruiqiong 1 Wang Guoping 2 Tang Guozhong 2 Wu Yuxin 3 Zhu Chengmo 3 Cao Shilong 1 1. Preparation of 125 I-Tumor necrosis factor mutants by iodogen and the measurement of its specific radioactivity[J]. Nuclear techniques, 1997, (5). DOI：

摘要

用Ｉｏｄｏｇｅｎ法对肿瘤坏死因子突变体进行碘标记，用放射纸层析法及自身取代分析法对产物的放化纯度及比放射性活度进行了计算。结果表明：碘的利用率为７２．７６％，纯化后标记物的游离碘为２．１２％，放化纯度为９３．１４％，比放射性活度为１．２ＴＢｑ／ｇ。标记后的肿瘤坏死因子突变体的细胞毒效应只下降４．３８％，提示Ｉｏｄｏｇｅｎ法标记肿瘤坏死因子突变体可获良好效果。

Abstract

Tumor necrosis factor mutants（TNF-m） was iodolabelled with iodogen and the radiochemical purity and specific radioactivity were examined with radio- paper chromatography and self-displacement analysis

The results showed: the utilization ratio of iodine was 72.76%; the free iodine of the 125 I-TNF-m after purification was 2.12%; the radiochemical puritiy of the 125 I-TNF-m was 93.14%; the specific radioactivity was 1.2TBq/g and the cytotoxicity rate of 125 I-TNF-m was only 4.38% lower than that of TNF-m. It could be concluded that labelling of TNF-m by iodogen results well.

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