Fluorescence microscopic and microautoradiographic observations on  apoptosis in immune cells induced by internal irradiation of 147  Pm

Zhu Shoupeng  Zhang Lansheng  Fu Qiang

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Fluorescence microscopic and microautoradiographic observations on apoptosis in immune cells induced by internal irradiation of 147 Pm

更新时间：2021-04-09

- Fluorescence microscopic and microautoradiographic observations on apoptosis in immune cells induced by internal irradiation of 147 Pm
- NUCLEAR TECHNIQUES Issue 11, (1997)
- 作者机构：
  
  苏州医学院
- 作者简介：
- 基金信息：
- DOI：
  CLC： TH742.65
- Published：01 November 1997
- 稿件说明：
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[1]朱寿彭,张澜生,付强.荧光显微镜及放射自显影观察 ~(147)Pm 内照射诱发免疫细胞凋亡[J].核技术,1997(11):23-26.

Zhu Shoupeng Zhang Lansheng Fu Qiang. Fluorescence microscopic and microautoradiographic observations on apoptosis in immune cells induced by internal irradiation of 147 Pm[J]. Nuclear techniques, 1997, (11).
[1]朱寿彭,张澜生,付强.荧光显微镜及放射自显影观察 ~(147)Pm 内照射诱发免疫细胞凋亡[J].核技术,1997(11):23-26. DOI：

Zhu Shoupeng Zhang Lansheng Fu Qiang. Fluorescence microscopic and microautoradiographic observations on apoptosis in immune cells induced by internal irradiation of 147 Pm[J]. Nuclear techniques, 1997, (11). DOI：

摘要

对荧光涂料１４７Ｐｍ内照射人淋巴细胞白血病细胞株Ｍｏｌｔ－４细胞和巨噬细胞株ＡＮＡ－１细胞诱发细胞凋亡进行了研究。估算了１４７Ｐｍ在不同阶段培养细胞中的辐射累积吸收剂量。通过荧光显微镜的形态观察，发现免疫细胞Ｍｌｔ－４和ＡＮＡ－１细胞在受１４７Ｐｍ内照射作用后，可诱发明显的核断裂和核固缩，以及凋亡小体形成为特征的细胞凋亡。微观放射自显影研究表明，１４７Ｐｍ可通过免疫细胞膜，在细胞内呈亲膜性积聚，并在膜包裹的凋亡小体周围呈现。从而证明了１４７Ｐｍ内照射可导致免疫细胞Ｍｏｌｔ－４和ＡＮＡ－１细胞的凋亡发生。

Abstract

? In this paper

apoptosis in human acute lymphoblastic leukemia cell line Molt-4 cell and macrophage cell line ANA-1 cell were induced by fission product 147 Pm. The cumulative absorption dose of 147 Pm in cultural cells through different periods were estimated. The morphological changes in Molt-4 and ANA-1 cells were observed by fluorescence microscopy and microautoradiographic tracing. Molt-4 and ANA-1 cells internally irradiated by 147 Pm were found

displayed a significant nuclear fragmentation and a marked pyknosis in immune cell’s nuclei

as will as DNA chain fragmentation and apoptotic bodies formation. The microautoradiographic study showed that 147 Pm could penetrate cell membrane and displayed membrane-seeking condensation in cells. At the same time

the membrane-bounded apoptotic bodies were observed. Experimental results in recent study provide evidence that Molt-4 and ANA-1 immune cells undergo apoptosis while internally irradiated with 147 Pm.

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