Observation of immunogold labelled tumor cell surface antigens by soft X-ray contact microscopy

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Observation of immunogold labelled tumor cell surface antigens by soft X-ray contact microscopy

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- Observation of immunogold labelled tumor cell surface antigens by soft X-ray contact microscopy
- NUCLEAR TECHNIQUES Issue 1, (1996)
- 作者机构：
  
  1. 安徽医科大学
  2. 中国科学院高能物理研究所同步辐射国家重点实验室
  3. 安徽医科大学中国科学院高能物理研究所同步辐射国家重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R73-34
- Published：01 January 1996
- 稿件说明：
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[1]魏道严,沈恒嘉,徐文轩,唐鄂生.免疫金标记肿瘤细胞表面抗原的软X射线接触显微术观察[J].核技术,1996(01):41-43.

Observation of immunogold labelled tumor cell surface antigens by soft X-ray contact microscopy[J]. Nuclear techniques, 1996, (1).
[1]魏道严,沈恒嘉,徐文轩,唐鄂生.免疫金标记肿瘤细胞表面抗原的软X射线接触显微术观察[J].核技术,1996(01):41-43. DOI：

Observation of immunogold labelled tumor cell surface antigens by soft X-ray contact microscopy[J]. Nuclear techniques, 1996, (1). DOI：

摘要

在建立了适于软Ｘ射线接触显微术（ＳＸＣＭ）观察的细胞样品免疫细胞化学染色技术的基础上，对培养的Ｈｅｏ—２肿瘤细胞表面ＨＬＡ—Ａ抗原进行免疫金银染色，并应用ＳＸＣＭ观察．结果显示：肿瘤细胞表面抗原的免疫金银染色颗粒图像反差明显，立体感强，大小和形态各异的颗粒清晰易辨．

Abstract

After establishing the immuno-cytochemical staining technique of cell samples suitable for soft X-ray contact microscopy（SXCM）

the HLA-A2 surface antigens of cultured Hep-2 tumor cells were labelled by immunogold silver staining（IGSS） and detected by SXCM. The resultS reveal that the immunogold silver granules on the surface of Hep-2 tumor cells have sharp contrast and show stereo features

and the granules with different sizes and shapes can be clearly seen and easily identified.

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