the technique of free radical probe i.e.the method of determining Bragg peak location of heavy ion beam in wheat seeds using ESR spectroscopy
has been put forward in the paper. It is shown that Bragg peak of 21MeV/u 14N7+ ion beam is located in wheat embryos with an implanted depth of less than 1.0±0.2mm while 48MeVlu 14N7+ ion beam can penetrate the whole wheat seed
and hence the latter can not be used for site-chosen mutation.