A microwave method reconstructing 99mTc-sestamibi was reported.The optimum condition was heating 15s at medium(940W) pewer in a microwave oven. The radiochemical purity of the 99mTc-sestamibi was over 95%
measured by a γ counter.The recommended thin-layer chromatography system involved. the use of a polyamide-coated plate and acetonitrile as developing solvent. The microwave ovenheating method will facilitate the emergency use of 99mTc-sestamibi.