Establishment of radioligand binding assay of gastrin receptors

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Establishment of radioligand binding assay of gastrin receptors

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- Establishment of radioligand binding assay of gastrin receptors
- NUCLEAR TECHNIQUES Issue 11, (1996)
- 作者机构：
  
  上海医科大学附属华山医院
- 作者简介：
- 基金信息：
- DOI：
  CLC： R817.45
- Published：01 November 1996
- 稿件说明：
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[1]黄广建,邓守真,乐竹琴,张延龄,王德昭,余枫.胃泌素受体放射配基结合分析法的建立[J].核技术,1996(11):653-656.

Establishment of radioligand binding assay of gastrin receptors[J]. Nuclear techniques, 1996, (11).
[1]黄广建,邓守真,乐竹琴,张延龄,王德昭,余枫.胃泌素受体放射配基结合分析法的建立[J].核技术,1996(11):653-656. DOI：

Establishment of radioligand binding assay of gastrin receptors[J]. Nuclear techniques, 1996, (11). DOI：

摘要

建立了胃泌素受体（ＧＲ）的放射配基结合分析法，测定３４例胃癌及邻近正常粘膜胃泌素受体含量及亲和力Ｋｄ。发现五肽胃泌素（Ｇｓ－５）及［Ｌｅｕ１５］Ｇｓ－１７均能作为非标记配基，Ｇｓ－５的抑制效果更好。醋酸纤维混合膜（Φ０．４５μｍ）分离结合及游离部分１２５［Ｌｅｕ１５］Ｇｓ－１７效果好。３４例胃癌及其邻近正常粘膜表达ＧＲ是一致的。

Abstract

Radioligand binding assay of gastrin receptor（GR） was established to determine GR content and Kd of gastric cancer and mucosa in specimens of 34 patients. Both of Gs-5 and [len15]Gs-17

as unlabelled ligand

can inhibit the binding of 125I-（leu15）Gs-17to the membrane

and the inhibiting effect of Gs-5 is slightly stronger than that of （len15）Gs-17.Mixed cellulose acetate filters have better effect to separate bound 125I （leu15）Gs-17 from free 125I- （len15）Gs-17 than glass fibre filters （Hongguan 999999）.The GR of gastric cancer correlates with that of mucosa in the same region of 34patients.

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