Rapid determination of the radiochemical purity of ￣（99m）Tc-HMPAO by mini-paper chromatography

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Rapid determination of the radiochemical purity of ￣（99m）Tc-HMPAO by mini-paper chromatography

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- Rapid determination of the radiochemical purity of ￣（99m）Tc-HMPAO by mini-paper chromatography
- NUCLEAR TECHNIQUES Issue 11, (1995)
- 作者机构：
  
  上海医科大学附属华山医院
- 作者简介：
- 基金信息：
- DOI：
  CLC： R811.3
- Published：01 November 1995
- 稿件说明：
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[1]薛方平,顾兆祥,林祥通.小纸层析法快速测定~（99m）Tc—HMPAO放射化学纯度[J].核技术,1995(11):690-692.

Rapid determination of the radiochemical purity of ￣（99m）Tc-HMPAO by mini-paper chromatography[J]. Nuclear techniques, 1995, (11).
[1]薛方平,顾兆祥,林祥通.小纸层析法快速测定~（99m）Tc—HMPAO放射化学纯度[J].核技术,1995(11):690-692. DOI：

Rapid determination of the radiochemical purity of ￣（99m）Tc-HMPAO by mini-paper chromatography[J]. Nuclear techniques, 1995, (11). DOI：

摘要

采用小纸层析系统和１：１的氯仿／四氢呋喃展开剂分析９９ｍＴｃ－ＨＭＰＡＯ的放射化学纯度，并与两个薄层层析系统和一个纸层析系统的方法作比较，配对ｔ检验结果表明两种方法测定的放射化学纯度无显著差异（Ｐ＞０．０５）；放射化学纯度在７４．２％－９６．４％（ｎ＝３０），小纸层析法与薄层／纸层析法紧密相关（ｒ＝０．９７，Ｐ＜０．００１），回归公式ｙ＝１．００６ｘ－０．３２３．

Abstract

A mini-paper chromatography （MPC） system has been developed to analyze the radiochemical purity of 99mTc-HMPAO involving a 1:1 chloroform/tetrahydrofuran developing solvent. The MPC procedure is compared with the method of two thin layer chromatographic system and one paper chromatographic system （TLC/PC）. The results of the paired t-tests show that radiochemical purities measured by MPC and TLC/PC do not difference significantly （P>0.05）. For radiochemical purity values ranging from 74.2%- 96.4% （n=30）

the MPC and TLC/PC methods correlated closely （r= 0.97） with a regression equation of MPC （%） = 1.006 TLC/PC-0.323.

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