Preliminary study on effects Of nitrogen ion implantation on corn（Zea mays）somatic cell culture

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Preliminary study on effects Of nitrogen ion implantation on corn（Zea mays）somatic cell culture

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- Preliminary study on effects Of nitrogen ion implantation on corn（Zea mays）somatic cell culture
- NUCLEAR TECHNIQUES Issue 7, (1995)
- 作者机构：
  
  1. 沈阳农业大学
  2. 大连理工大学
  3. 辽宁省开原种子公司
  4. 铁岭市干部培训中心
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q943
- Published：01 July 1995
- 稿件说明：
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[1]刘世强,贾景明,曹萍,杨吉存,张宝石,刘景荣,高萍,史维东.氮离子束注入对玉米体细胞培养影响的初步研究[J].核技术,1995(07):410-414.

Preliminary study on effects Of nitrogen ion implantation on corn（Zea mays）somatic cell culture[J]. Nuclear techniques, 1995, (7).
[1]刘世强,贾景明,曹萍,杨吉存,张宝石,刘景荣,高萍,史维东.氮离子束注入对玉米体细胞培养影响的初步研究[J].核技术,1995(07):410-414. DOI：

Preliminary study on effects Of nitrogen ion implantation on corn（Zea mays）somatic cell culture[J]. Nuclear techniques, 1995, (7). DOI：

摘要

将能量为６０ｋｅＶ、剂量为２×１０１６Ｎ＋／ｃｍ２的氮离子束分别注入３个玉米自交系的成熟种子．从其Ｍ１成株上取下未成熟的胚作外植体，在ＭＮ和ＡＧ培养基上进行高体培养，以未注入的相同材料为对照．结果发现，Ｍ１的平均愈伤组织诱导频率比对照高１１．７％，其中在ＭＮ和ＡＧ培养基上，分别比对照高１５．５％和７．９％、不同类型的材料，在两种培养基上的愈伤组织诱导频率存在差异，而且在诱导过程中Ｍ１与对照材料均要求６－ＢＡ的浓度在０．５－１．５ｍｇ／Ｌ。

Abstract

Immature embryos from three self-bred series of M1 corn（Zea mays）were cultured in MN and AG medium after dry seeds were implanted to 2×1016cm-2 N+ ions of 60keV and grown into M1 plants. The experiments indicated that all genotypes formed pale-yellow or yellow callus in the two media. The average callus induction frequency of M1 immature embryos was 11.7% higher than that of the control. Percentages of callus were different not only for various genotypes of the culture material in the two media

but also for different concentration of 2

4-D and 6-BA.

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