A study on the incubation time required for solving receptor parameters with multi-point radioligand binding assay

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A study on the incubation time required for solving receptor parameters with multi-point radioligand binding assay

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- A study on the incubation time required for solving receptor parameters with multi-point radioligand binding assay
- NUCLEAR TECHNIQUES Issue 11, (1994)
- 作者机构：
  
  1. 上海第二医科大学
  2. 上海医科大学
  3. 上海第二医科大学上海医科大学
- 作者简介：
- 基金信息：
- DOI：
  CLC： R815
- Published：01 November 1994
- 稿件说明：
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[1]向荣,易宁育,夏宗勤.用多点放射配基结合分析法求解受体参数所需孵育时间的研究[J].核技术,1994(11):684-687.

A study on the incubation time required for solving receptor parameters with multi-point radioligand binding assay[J]. Nuclear techniques, 1994, (11).
[1]向荣,易宁育,夏宗勤.用多点放射配基结合分析法求解受体参数所需孵育时间的研究[J].核技术,1994(11):684-687. DOI：

A study on the incubation time required for solving receptor parameters with multi-point radioligand binding assay[J]. Nuclear techniques, 1994, (11). DOI：

摘要

对受体的放射配基结合实验中反应时间与配基浓度的关系进行了数学分析和实验研究，证明当其它条件固定时，反应达到平衡所需的时间是放射配基初始浓度的函数。一般是初始浓度越小，平衡时间越长；用多点放射配基结合分析法求解受体参数时．孵育时间应选择能使低浓度放射配基达到平衡的时间，对可能出现的“倒钩”现象进行了讨论。

Abstract

Mathematical analysis and kinetic experiments demonstrated that the incubation timet required for achieving equilibrium in radioligand binding assays of receptors varies with the total concentration of radioligand LT. With other conditions kept constant

the smaller the radioligand concentration

the longer the take requied.Therefore

the incubation time should be determined by the time required for achieving equilibrium with the lowest instead of higher concentrations of radioligand. The occassional "hook" phenomenon of the t-LT correlation curve at very low doses of LT has been discussed.

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