Direct labelling of monomeric antibody fragments Fab’with￣（99m）Tc

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Direct labelling of monomeric antibody fragments Fab’with￣（99m）Tc

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- Direct labelling of monomeric antibody fragments Fab’with￣（99m）Tc
- NUCLEAR TECHNIQUES Issue 11, (1994)
- 作者机构：
  
  中国医学科学院北京协和医院
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392
- Published：01 November 1994
- 稿件说明：
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[1]李军,王世真,杨子义.抗体单价片段Fab＇的~（99m）Tc直接标记[J].核技术,1994(11):650-652.

Direct labelling of monomeric antibody fragments Fab’with￣（99m）Tc[J]. Nuclear techniques, 1994, (11).
[1]李军,王世真,杨子义.抗体单价片段Fab＇的~（99m）Tc直接标记[J].核技术,1994(11):650-652. DOI：

Direct labelling of monomeric antibody fragments Fab’with￣（99m）Tc[J]. Nuclear techniques, 1994, (11). DOI：

摘要

研究了用９９ｍＴｃ直接标记抗体单价片段Ｆａｂ’的方法与条件．ＩｇＧ型多抗经无花果蛋白酶消化后得到Ｆ（ａｂ＇）２双价片段，再经２－巯基乙胺还原为Ｆａｂ＇单价片段，与９９ｍＴｃ及亚锡葡萄糖酸钠药盒溶液混合保温，即可得到９９ｍＴｃ－Ｆａｂ’标记物，标记率为８５％－９５％。这标记物在小鼠血中的清除速率明显高于完整抗体标记物９９ｍＴｃ－ＩｇＧ．

Abstract

irect labelling method and conditions of monomeric antibody Fab’ with 99mTc were investigated. Polyclonal antibody IgG was digested with ficin to produce dimeric fragments F（ab’）2

which was subsequently reduced to monomeric fragments Fab’ with 2-mercaptoetylamine. Finally

Fab’ was incubated with sodium gluconate（Sn（Ⅱ）） kit solution and 99mTc

eluated at room temperature to form 99mTc-Fab’.The labelling efficiency was 85%- 95%. The stability of labelled products was satisfactory and the elimination rate was faster than 90mTc-IgG.

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