Study on fluorescence-sensitization of 3 variations of scintillators in high speed autoradiography

Wang Zhenli Liu Guimin

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Study on fluorescence-sensitization of 3 variations of scintillators in high speed autoradiography

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- Study on fluorescence-sensitization of 3 variations of scintillators in high speed autoradiography
- NUCLEAR TECHNIQUES Issue 5, (1993)
- 作者机构：
  
  1. 天津医药科学研究所
  2. 天津医学院
  3. 天津南开医院
  4. 北京医科大学,天津,300070
  5. ,北京,100083
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：01 May 1993
- 稿件说明：
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[1]王振立,刘桂敏,黄凤岐,王民宪,刘鼎新.三种闪烁体在高速自显影中的荧光增敏效率的研究[J].核技术,1993(05):303-305.

Wang Zhenli Liu Guimin. Study on fluorescence-sensitization of 3 variations of scintillators in high speed autoradiography[J]. Nuclear techniques, 1993, (5): 303-305.
[1]王振立,刘桂敏,黄凤岐,王民宪,刘鼎新.三种闪烁体在高速自显影中的荧光增敏效率的研究[J].核技术,1993(05):303-305. DOI：

Wang Zhenli Liu Guimin. Study on fluorescence-sensitization of 3 variations of scintillators in high speed autoradiography[J]. Nuclear techniques, 1993, (5): 303-305. DOI：

摘要

在

H-TdR细胞参入试验、

H-TdR低高浓度两次细胞标记及细胞迁移示踪试验中

对三种闪烁体POPOP、PBD及PPO的不同浓度的荧光增敏效率进行了比较

结果表明

7.5％PPO的二甲苯闪烁液在样品曝光48h后增敏效果最佳

灵敏度比普通方法提高15-20倍

为提高显微自显影的效率提供了依据。

Abstract

The sensitizing effects of POPOP

PBD and PPO were compared in 3H-TdR incorporation experiment

3H-TdR low and high concentration twice labelling experiment and a cell migration tracer experiment. The results indicate that 7.5% PPO in xylene with an exposure time of 48 h is most satisfactory. The efficiency was increased for 15-20 times.

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