The establishment of [<sup>3</sup>H]-Sulpiride radioligand binding assay

Wang Qiang Zhu Tong Zhu Gengen Shen Chengwu

您当前的位置：

首页 >

文章列表页 >

The establishment of [³H]-Sulpiride radioligand binding assay

更新时间：2021-04-09

- The establishment of [³H]-Sulpiride radioligand binding assay
- NUCLEAR TECHNIQUES Issue 5, (1993)
- 作者机构：
  
  1. 上海医科大学
  2. 上海医科大学,上海,200032
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：01 May 1993
- 稿件说明：
移动端阅览
[1]王蔷,朱桐,朱根根,沈承武.氚标舒必利放射配基结合分析法的建立[J].核技术,1993(05):271-274.

Wang Qiang Zhu Tong Zhu Gengen Shen Chengwu. The establishment of [³H]-Sulpiride radioligand binding assay[J]. Nuclear techniques, 1993, (5): 271-274.
[1]王蔷,朱桐,朱根根,沈承武.氚标舒必利放射配基结合分析法的建立[J].核技术,1993(05):271-274. DOI：

Wang Qiang Zhu Tong Zhu Gengen Shen Chengwu. The establishment of [³H]-Sulpiride radioligand binding assay[J]. Nuclear techniques, 1993, (5): 271-274. DOI：

摘要

研究了氚标舒必利（[

H]-Sulpiride）与大鼠纹状体膜蛋白的结合特性

结果表明两者结合具有特异性、饱和性、可逆性和配体选择性

max

=917.0±133.2pmol/g蛋白

=5.17±1.13nmol/l;并对根据这一结合特性建立的[

H]-Sulpiride放射配基结合分析法作为测定血中舒必利浓度的方法进行分析

该方法的线性范围是1—100nmol/l

灵敏度是49.25ng/l

批内、批间变异系数分别为3.35％和10.6％。

Abstract

Characteristics of [3H]- Sulpiride binding in rat striatal membranes by ligand binding assay were analyzed. The results showed that the binding is specific

saturable

reversible and selective. Scatchard analysis of the data revealed a single binding component with a dissociation constant （Kd） of 5.17±1.13nmol/l （X±SD

n = 5） and a maximum binding capacity （Bmax） of 917.0±133.2fmol/mg protein （X±SD

n = 5）. Based on the ligand binding assay

[3H]- Sulpiride radioligand assay has been developed. The linear range of the assay was 1-100nmol/l. The sensitivity was 49.25pg/ml. The coefficients of intra- and interassay variation were 3.35% （n = 10） and 10.6% （re = 4）

respectively.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

The study of the androgen receptor profile and changes of level of serum testosterone in human prostatic cancer

The assay of estrogen receptors in three components of human breast cancer tissue

Related Author

Gui Zhining Hu Xiaoke Lu Hanping Fan WeiWu Naiyun Gao Jinhui

胡笑克

卢汉平

樊卫

吴乃允

高锦辉

梅骅

曾金云

Related Institution

Sun Yat -sen University of Medical Sciences, Guangzhou 510089)Mei Hua Zeng Jinyun

中山医科大学附属一院

510089广州市红十字会医院

510089中山医科大学肿瘤医院

AI问答

Address：2019 Jialuo Road, Jiading, Shanghai, China Postal code：201800
Tel：021-39194977 Email：LHB@sinap.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 沪ICP备05005479号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
Total Visits：880771 Visits Today：298

⁰

The establishment of [3H]-Sulpiride radioligand binding assay

DOI：

摘要

Abstract

关键词

Keywords

references

The establishment of [³H]-Sulpiride radioligand binding assay