A study on the immunoactivity of <sup>2</sup>11At -antigastric cancer McAb3H<sub>1</sub>1 and its injury effect on gastric cancer cells

Wang Juan Zhou Maolun Jin Jiannan Zhang Shuyuan Liu Ning Luo Lin Wang Fangyuan

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A study on the immunoactivity of ²11At -antigastric cancer McAb3H₁1 and its injury effect on gastric cancer cells

更新时间：2021-04-09

- A study on the immunoactivity of ²11At -antigastric cancer McAb3H₁1 and its injury effect on gastric cancer cells
- NUCLEAR TECHNIQUES Issue 11, (1992)
- 作者机构：
  
  1. 四川大学原子核科学技术研究所
  2. 北京肿瘤防治研究所,成都,610064
  3. ,北京,100034
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：01 November 1992
- 稿件说明：
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[1]王娟,周懋伦,金建南,张叔渊,刘宁,罗林,王方元,万文徽,董志伟.~(211)At-3H_(11)McAb的免疫活性及体外杀伤效应研究[J].核技术,1992(11):678-682.

Wang Juan Zhou Maolun Jin Jiannan Zhang Shuyuan Liu Ning Luo Lin Wang Fangyuan. A study on the immunoactivity of ²11At -antigastric cancer McAb3H₁1 and its injury effect on gastric cancer cells[J]. Nuclear techniques, 1992, (11): 678-682.
[1]王娟,周懋伦,金建南,张叔渊,刘宁,罗林,王方元,万文徽,董志伟.~(211)At-3H_(11)McAb的免疫活性及体外杀伤效应研究[J].核技术,1992(11):678-682. DOI：

Wang Juan Zhou Maolun Jin Jiannan Zhang Shuyuan Liu Ning Luo Lin Wang Fangyuan. A study on the immunoactivity of ²11At -antigastric cancer McAb3H₁1 and its injury effect on gastric cancer cells[J]. Nuclear techniques, 1992, (11): 678-682. DOI：

摘要

以间接标记法首先合成中间体对砹苯甲酸

再通过活化羧基反应联接到3H

McAb上

制得

211

At-3H

McAb偶联物

放射性比度为18.5—61.1kBq/μg。ELISA法鉴定标记后的

211

At-3H

McAb的免疫活性与未标记3H

McAb一致。实验表明:

211

At-3H

McAb对靶细胞杀伤效应高于

211

At-IgG及Na

211

At;当靶细胞先与一定量3H

McAb或3G

McAb（4μg）作用1h

可抑制

211

At-3H

McAb对靶细胞的杀伤效应

放射性比度不影响杀伤效应。

Abstract

The synthesis of 211 At labelled 3H11 monoclonal antibody was performed by an activation reaction of carboxy via paraastato - benzoic acid intermediate. Its specific radioactivity was 18. 5-61. 1kBq/μg . Its immunoactivity was measured by ELISA method. In vitro binding and cytotoxic experiments showed when the 211At - 3H11McAb concentration is raised

the cytotoxic effect increased and have obvious selectivity. The cytotoxic effect of 211At - 3H11 McAb to target cell M85 was higher than that of 211At - IgG and Na211At . If the M85 cells were cultured with 3H11 McAb or 3G9McAb for 1 h at first

the cytotoxic effect of 211At -3H11McAb was inhibited. The specific radioactivity of 211At - 3H11McAb and cytotoxic effect are also discussed.

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A study on the immunoactivity of 211At -antigastric cancer McAb3H11 and its injury effect on gastric cancer cells

DOI：

摘要

Abstract

关键词

Keywords

references

A study on the immunoactivity of ²11At -antigastric cancer McAb3H₁1 and its injury effect on gastric cancer cells