To explore whether synchrotron radiation hard X-ray has any effects on biological tissue structure and re- lated antigen expression of fixed bone

cartilage

liver and lung

diffraction-enhanced X-ray imaging (DEI) and in-line holography with synchrotron radiation hard X-ray from Beijing Synchrotron Radiation Facility (BSRF) were applied to observe the morphology of the bone defect of rabbit

the lung cancer of mouse

the hepatic fibrosis and hepatocir- rhosis of SD rat as well as their respective control. The histologic changes were observed by microscope. The semi- quantitative analysis of antigen-III

S-100

FN and ubiquitin were also performed by immunohistochemistry. Imaged tissue was found to be intact

and there was no difference in these antigen expressions between the imaged and control groups. It is concluded that synchrotron radiation hard X-ray has no effect on biological tissue structure and related antigen expression of fixed bone

cartilage

liver and lung. The present results have provided experimental evidences for the development of synchrotron hard X-ray-phase technique of in vitro biological and medical studies.