Changes of cell-cycle progression of different types of tumor cell lines induced by 3Gy γrays

XIA Jingguang1; 2 LI Wenjian1 WEI Wei1; 2 ZHAO Jing1; 2 JIN Xiaodong1; 2 GAO Qingxiang3 1

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Changes of cell-cycle progression of different types of tumor cell lines induced by 3Gy γrays

更新时间：2021-03-12

- Changes of cell-cycle progression of different types of tumor cell lines induced by 3Gy γrays
- NUCLEAR TECHNIQUES Issue 8, (2005)
- 作者机构：
  
  1. 中国科学院近代物理研究所
  2. 兰州大学生命科学学院兰州730000中国科学院研究生院北京,100049
  3. ,兰州,730000
- 作者简介：
- 基金信息：
  
  国家自然科学基金重点项目(10335050);国家重大基础研究前期研究专项项目(2003CCB00200)资助
- DOI：
  CLC： R73-3
- Published：01 August 2005
- 稿件说明：
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[1]夏景光,李文建,魏巍,赵靖,金晓东,高清祥.3Gyγ射线对不同肿瘤细胞细胞周期进程的影响[J].核技术,2005(08):621-624.

XIA Jingguang1, 2 LI Wenjian1 WEI Wei1, 2 ZHAO Jing1, et al. Changes of cell-cycle progression of different types of tumor cell lines induced by 3Gy γrays[J]. Nuclear techniques, 2005, (8): 621-624.
[1]夏景光,李文建,魏巍,赵靖,金晓东,高清祥.3Gyγ射线对不同肿瘤细胞细胞周期进程的影响[J].核技术,2005(08):621-624. DOI：

XIA Jingguang1, 2 LI Wenjian1 WEI Wei1, 2 ZHAO Jing1, et al. Changes of cell-cycle progression of different types of tumor cell lines induced by 3Gy γrays[J]. Nuclear techniques, 2005, (8): 621-624. DOI：

摘要

本实验旨在研究电离辐射对不同肿瘤细胞细胞周期进程的影响

为肿瘤的放射治疗提供科学依据。以3Gyγ射线照射指数生长期的肿瘤细胞

用流式细胞术测定细胞DNA含量并确定细胞周期各时相的细胞百分比。实验结果显示

γ射线照射后

SMMC-7721

hepG2和HO-8910细胞的G2/M期发生明显阻滞

且都在辐射后12h累积达到最大值

但S期只发生短暂的延迟

即辐射后6h明显累积

之后下降至接近对照水平或低于对照;Hela细胞的G2/M期和S期均发生显著阻滞。实验结果说明

电离辐射后

不同肿瘤细胞的G2/M期和S期检查点的激活和维持是有差异的。

Abstract

Exponentially growing human tumor cell lines were γ -irradiated with a dose of 3Gy. The DNA content and percentage of each cell-cycle phase were obtained with flow cytometry. Results indicated that for SMMC-7721

hepG2 and HO-8910 cells

G2/M accumulation was prominently induced by irradiation and the maximal accumulation appeared at 12 h after treatment

and that S phase just displayed temporal accumulation

i.e. S phase cells accumulated at 6 h after irradiation

and thereafter decreased close to or below the level of un-irradiated cells

and that for Hela cells both G2/M and S phase accumulation were pronouncedly triggered by irradiation. It is suggested that there remain differences in activation and maintenance of cell cycle checkpoints after irradiation among these four tumor cell lines.

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Related Institution

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Nanjing University of Aeronautics and Astronautics

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