Effect of sargassum hemiphyllum polysaccharides on splecocytes in irradiated mice

MENG Qingyong LIU Zhihui XU Meiyi

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Effect of sargassum hemiphyllum polysaccharides on splecocytes in irradiated mice

更新时间：2021-03-12

- Effect of sargassum hemiphyllum polysaccharides on splecocytes in irradiated mice
- NUCLEAR TECHNIQUES Issue 7, (2005)
- 作者机构：
  
  1. 广东医学院分析中心
  2. 广东医学院分析中心,湛江,524023
- 作者简介：
- 基金信息：
  
  广东省湛江市科技重点攻关项目(9945)
- DOI：
  CLC： R285
- Published：01 July 2005
- 稿件说明：
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[1]孟庆勇,刘志辉,徐美奕.半叶马尾藻多糖对辐射损伤小鼠脾细胞的影响[J].核技术,2005(07):542-545.

MENG Qingyong LIU Zhihui XU Meiyi. Effect of sargassum hemiphyllum polysaccharides on splecocytes in irradiated mice[J]. Nuclear techniques, 2005, (7): 542-545.
[1]孟庆勇,刘志辉,徐美奕.半叶马尾藻多糖对辐射损伤小鼠脾细胞的影响[J].核技术,2005(07):542-545. DOI：

MENG Qingyong LIU Zhihui XU Meiyi. Effect of sargassum hemiphyllum polysaccharides on splecocytes in irradiated mice[J]. Nuclear techniques, 2005, (7): 542-545. DOI：

摘要

探讨了半叶马尾藻多糖[Sargassumhemiphyllum（Turner）C.Ag.polysaccharides

SHP]对辐射损伤小鼠脾细胞的影响。用3H-TdR（氚标记胸腺嘧啶核苷）和3H-TyR（氚标记酪氨酸）掺入法分别检测脾细胞DNA、UDS（非程序性DNA合成）和蛋白质合成

通过流式细胞术测定脾细胞周期和脾淋巴细胞CD4分子表达。20mg/kg和40mg/kg的SHP对辐射损伤小鼠DNA和蛋白质合成具有显著的刺激作用

10—40mg/kg的SHP对辐射损伤小鼠脾淋巴细胞UDS和CD4分子的表达也有明显的促进作用。脾细胞周期研究表明20mg/kg和40mg/kg的SHP对辐射损伤小鼠脾细胞G0/G1期和G2/M期的细胞百分率分别有减少和增加的作用

而10—40mg/kg的SHP能够提高辐射损伤小鼠脾细胞S期的细胞百分率。SHP对辐射损伤小鼠脾细胞具有保护作用。

Abstract

To evaluate the effect of SHP [Sargassum hemiphyllum (Turner) C.Ag. polysaccharides] on splenocytes in irradiated mice

the UDS

DNA and protein’s synthesis of murine splenocytes were detected by 3H-TdR and 3H-TyR incorporation test. Flow cytometry was used to determine the distribution of cell cycles and CD4 expression. The SHP (20 mg/kg and 40 mg/kg) stimulated the synthesis of DNA and proteins in splenocytes of irradiated mice. The SHP (10 —40 mg/kg) significantly enhanced the UDS and CD4 expression of splenocytes in irradiated mice. The SHP (20 mg/kg and 40 mg/kg) might induce the change of cell cycles of splenocytes in irradiated mice; the percentage of cells was decreased in G0/G1 phase and increased in G2/M phase. However

the percentage of cells in S phase for the SHP (10 —40 mg/kg) group was significantly higher than that for the control group. Accordingly

the SHP has a protective effect on splenocytes in irradiated mice.

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