Predict of radiosensitivity of human hepatoma cells irradiated with γ-rays by G2 PCC technique

YANG Jianshe1; 2 ZHAO Jing1; 2 LI Wenjian1 GAO Qingxiang3 WANG Zhuanzi1; 2 1

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Predict of radiosensitivity of human hepatoma cells irradiated with γ-rays by G2 PCC technique

更新时间：2021-03-12

- Predict of radiosensitivity of human hepatoma cells irradiated with γ-rays by G2 PCC technique
- NUCLEAR TECHNIQUES Issue 11, (2005)
- 作者机构：
  
  1. 中国科学院近代物理研究所
  2. 兰州大学生命科学学院
  3. 中国科学院近代物理研究所兰州730000中国科学院研究生院北京,100049
  4. ,兰州,730000
- 作者简介：
- 基金信息：
  
  国家自然科学基金重点课题(编号:10335050);国家重大基础研究前期研究专项(2003CCB00200)资助
- DOI：
  CLC： R735.7
- Published：01 November 2005
- 稿件说明：
移动端阅览
[1]杨建设,赵靖,李文建,高清祥,王转子.G2期早熟染色体凝集技术预测肝癌细胞低LET射线辐射敏感性[J].核技术,2005(11):45-48.

YANG Jianshe1, 2 ZHAO Jing1, 2 LI Wenjian1 GAO Qingxiang3 WANG Zhuanzi1, et al. Predict of radiosensitivity of human hepatoma cells irradiated with γ-rays by G2 PCC technique[J]. Nuclear techniques, 2005, (11).
[1]杨建设,赵靖,李文建,高清祥,王转子.G2期早熟染色体凝集技术预测肝癌细胞低LET射线辐射敏感性[J].核技术,2005(11):45-48. DOI：

YANG Jianshe1, 2 ZHAO Jing1, 2 LI Wenjian1 GAO Qingxiang3 WANG Zhuanzi1, et al. Predict of radiosensitivity of human hepatoma cells irradiated with γ-rays by G2 PCC technique[J]. Nuclear techniques, 2005, (11). DOI：

摘要

用60Co产生的γ射线照射人肝癌细胞系SMMC-7721。以克隆形成法测定经照射后的细胞存活率

用化学诱导剂Calyculin-A诱导的早熟染色体凝集（Prematurechromosomecondensation

PCC）技术研究染色体损伤。结果显示G2期细胞内的染色单体和等点染色单体断裂数与照射剂量之间存在着线性相关性

染色单体断裂数与细胞存活率之间存在较好的线性相关性。表明辐射诱导的染色单体断裂可以作为预测SMMC-7721细胞内在辐射敏感性的指标

也可为临床诊断和治疗肝癌提供依据。

Abstract

To investigate the radiation response of hepatoma cells SMMC-7721 the G2 premature chromosome con- densation technique was used. SMMC-7721 cells ( human hepatoma cells ) were irradiated with 60Co γ -rays. Then cell killing was documented by a colony assay. Initial chromatid breaks were measured by counting the number of chromatid breaks and isochromatid breaks immediately after prematurely chromosome condensed by Calyculin-A. A dose-dependent increase in radiation-induced chromatid / isochromatid breaks was observed in G2 phase. A good relationship was found between cell survival and chromatid breaks. This study implied that low LET radia- tion-induced chromatid / isochromatid breaks can be possibly used as a good predictor of radiosensitivity of SMMC-7721 hepatoma cells

also it could be used to design radiotherapy schedule for liver cancer patients.

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