Effects of vacuum and implantation on wheat’s POD isozymes produced by low energy ion beam implantation were studied. The same as the control sample

activity of POD isozymes produced by vacuum was increasing with adding the culture time. Activities of POD isozymes of the samples treated with different vacuum time but having the same culture time were increasing with prolonging the vacuum time. Impantated by N+ of different doses

germination rate of wheat seeds had no changes. But sprouting length of the sample implanted by higher dose was evidently shorter than others. Low dose implantation could stimulate POD isozyme’s activity to increase

while high dose implantation could retard POD isozyme’s activity.