Study on REE bound water-soluble polysaccharides in plant

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Study on REE bound water-soluble polysaccharides in plant

更新时间：2021-03-12

- Study on REE bound water-soluble polysaccharides in plant
- NUCLEAR TECHNIQUES Issue 3, (1999)
- 作者机构：
  
  1. 中国科学院高能物理研究所
  2. 中国科学院核分析技术开放实验室!北京1０００８０
  3. 中国科学院核分析技术开放实验室!北京１０００８０
  4. 中国农业科学院研究生院
- 作者简介：
- 基金信息：
  
  国家自然科学基金
- DOI：
  CLC： Q50
- Published：01 March 1999
- 稿件说明：
移动端阅览
[1]王玉琦,郭繁清,许雷,陈红民,孙景信,曹国印.植物体中稀土元素与水溶性多糖结合状态的研究[J].核技术,1999(03):160-164.

Study on REE bound water-soluble polysaccharides in plant[J]. Nuclear techniques, 1999, (3).
[1]王玉琦,郭繁清,许雷,陈红民,孙景信,曹国印.植物体中稀土元素与水溶性多糖结合状态的研究[J].核技术,1999(03):160-164. DOI：

Study on REE bound water-soluble polysaccharides in plant[J]. Nuclear techniques, 1999, (3). DOI：

摘要

利用分子活化分析技术初步研究了天然植物铁芒萁叶中稀土元素与水溶性多糖的结合状态。铁芒萁叶经过提取和生化分离后，得到冷水溶性多糖和热水溶性多糖。将所得到的未脱蛋白多糖及脱蛋白后的多糖分别通过凝胶柱，洗脱液经比色法测定多糖吸收曲线，考马士亮兰Ｇ－２５０检测蛋白峰，并用中子活化法测定了多糖中Ｌａ、Ｃｅ、Ｎｄ、Ｓｍ、Ｅｕ、Ｔｂ、Ｙｂ和Ｌｕ的含量。研究结果表明，铁芒萁叶子中的稀土元素与多糖紧密结合。经分子量测定，与稀土元素结合的多糖为小分子量多糖，分子量为１×１０４－２ｘ１０４Ｄａｌｔｏｎ。

Abstract

The binding of REE with water-soluble polysaccharides (PSs) in leaves of fern Dicranopteris Dichotoma (DD) has been studied by molecular activation analysis.The cold-water-soluble and hot-water-soluble PSs in leaves of DD were obtained by using biochemical separation techniques. The PSs of non-deproteinization and deproteinization

were separated on Sephadex G-200 gel permeation chromatography.The absorption curves of elution for the PSs were obtained by colorimetry

and the proteins were detected using Coomassic brilliant G-250. Eight REEs (La

Yb and Lu) in these PSs were determined by instrumental neutron activation analysis. The results obtained show that the REEs are bound firmly with the water-soluble PSs in the plant. A measurement demonstrates that the PSs bound with REEs are mainly of smaller molecular weight (10

000 to 20

000 Dalton).

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