Improved ethanol fermentation of a yeast mutant by C-12 ion beam irradiation

LU Dong1; 2 WANG Ying1; 3 LIU Qingfang1; 2 WU Xin1; 2 WANG Jufang1 MA Shuang1 LI Wenjian1 1

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Improved ethanol fermentation of a yeast mutant by C-12 ion beam irradiation

更新时间：2021-03-05

- Improved ethanol fermentation of a yeast mutant by C-12 ion beam irradiation
- NUCLEAR TECHNIQUES Vol. 33, Issue 5, (2010)
- 作者机构：
  
  1. 中国科学院近代物理研究所
  2. 中国科学院研究生院
  3. 兰州大学
- 作者简介：
- 基金信息：
  
  甘肃省自然科学基金(096RJZA126)资助
- DOI：
  CLC： TQ223.122
- Published：01 May 2010
- 稿件说明：
移动端阅览
[1]陆栋,王颖,刘青芳,吴鑫,王菊芳,马爽,李文建.~(12)C离子束辐照对酵母发酵能力的影响[J].核技术,2010,33(05):350-353.

LU Dong1, 2 WANG Ying1, 3 LIU Qingfang1, et al. Improved ethanol fermentation of a yeast mutant by C-12 ion beam irradiation[J]. Nuclear techniques, 2010, 33(5): 350-353.
[1]陆栋,王颖,刘青芳,吴鑫,王菊芳,马爽,李文建.~(12)C离子束辐照对酵母发酵能力的影响[J].核技术,2010,33(05):350-353. DOI：

LU Dong1, 2 WANG Ying1, 3 LIU Qingfang1, et al. Improved ethanol fermentation of a yeast mutant by C-12 ion beam irradiation[J]. Nuclear techniques, 2010, 33(5): 350-353. DOI：

摘要

利用100MeV/u的12C6+离子束辐照酵母Saccharomyces cerevsiea YY

选育出一株高产突变菌株C03A

考察C03A发酵过程中不同温度、pH、糖汁浓度对发酵的影响。通过正交实验确定最佳发酵条件为:糖汁浓度24%、温度35℃、pH5.0。在10L发酵罐实验中

C03A发酵速率相对原始菌株高

36h发酵完全

比原始菌株缩短12h;发酵产酒率达到13.2%（V/V）

比原始菌株高1.6%（V/V）。

Abstract

The yeast Saccharomyces cerevsiea YY was irradiated with 100 MeV/u 12C6+ ion beams.After screening

we obtained the mutant strain C03A of high ethanol yield.The influence of fermentation temperature

pH and concentration of sugar on ethanol fermentation were studied.The range analysis and analysis of variance were applied for the result of orthogonal experiments.The optimal ethanol fermentation conditions are:fermentation temperature 35°C

pH value 5.0

and sugar concentration 24%.The results of fermentation in the 10 L bioreactor showed that the ethanol fermentation of the mutant strain could be completed in 36 hours

the production of ethanol was to 13.2%(V/V)

which means 12 hours faster and 1.6%(V /V) ethanol yield higher than original strain.

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