Role of DNA damage repair capacity in radiation induced adaptive response

YUAN Dexiao PAN Yan ZHAO Meijia CHEN Honghong SHAO Cunlin

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Role of DNA damage repair capacity in radiation induced adaptive response

更新时间：2021-03-12

- Role of DNA damage repair capacity in radiation induced adaptive response
- NUCLEAR TECHNIQUES Vol. 32, Issue 5, (2009)
- 作者机构：
  
  复旦大学放射医学研究所
- 作者简介：
- 基金信息：
  
  国家自然科学基金(30670629,30770644);教育部新世纪优秀人才支持计划(NCET-06-0365);上海市浦江人才计划(06PJ14012)资助
- DOI：
  CLC： Q345
- Published：01 May 2009
- 稿件说明：
移动端阅览
[1]袁德晓,潘燕,赵镁嘉,陈红红,邵春林.DNA损伤修复能力对辐射适应性反应的影响[J].核技术,2009,32(05):366-370.

YUAN Dexiao PAN Yan ZHAO Meijia CHEN Honghong SHAO Cunlin. Role of DNA damage repair capacity in radiation induced adaptive response[J]. Nuclear techniques, 2009, 32(5): 366-370.
[1]袁德晓,潘燕,赵镁嘉,陈红红,邵春林.DNA损伤修复能力对辐射适应性反应的影响[J].核技术,2009,32(05):366-370. DOI：

YUAN Dexiao PAN Yan ZHAO Meijia CHEN Honghong SHAO Cunlin. Role of DNA damage repair capacity in radiation induced adaptive response[J]. Nuclear techniques, 2009, 32(5): 366-370. DOI：

摘要

采用野生型的中国仓鼠卵母细胞（CHO-9）及其DNA损伤修复缺陷型细胞:EM-C11（DNA单链断裂修复缺陷）和XR-C1（DNA双链断裂修复缺陷）

首先进行低剂量（0.016Gy或0.08Gy）的初始照射

间隔4h或7h后再进行高剂量（1Gy）的攻击照射

然后检测细胞微核形成率和克隆形成率的变化。结果显示:当初始剂量为0.08Gy

间隔4h后再施以1Gy的攻击照射时

三株细胞只有野生型的CHO-9有辐射适应性反应产生;但当间隔时间延长到7h时

CHO-9和EM-C11均产生了辐射适应性反应。当把初始照射剂量降低为0.016Gy

间隔4h再施以1Gy的攻击照射时

三株细胞均产生了辐射适应性反应。表明辐射适应性反应不仅与初始照射剂量及间隔时间有关

还与DNA损伤修复密切相关。

Abstract

This work was to explore γ-ray induced radioadaptive response(RAR) in Chinese hamster ovary(CHO) cell lines of different DNA damage repair capacities.CHO-9 cells and the two repair-deficient strains

EM-C11(DNA single strand break repair deficient) and XR-C1(DNA double strand break repair deficient)

were irradiated with a priming dose of 0.08 Gy or 0.016 Gy.After 4 or 7 hours

they were irradiated again with a challenging dose of 1 Gy.The micronucleus induction and plating efficiency of the cells were assayed.Under 0.08 Gy priming dose and 4-h interval

just the CHO-9 cells showed RAR

while with the 7-h interval the CHO-9 and EM-C11 showed RAR

but XR-C1 did not.When the cells were pretreated with a lower priming dose of 0.016 Gy in a 4-h time interval

all the three cell lines showed RAR to subsequent 1 Gy irradiation.It can be concluded that RAR is not only related to the priming dose and time interval

but also has close dependence on the ability of DNA damage repair.

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