Labeling monoclonal antibody 10D9 with <sup>125</sup>I using the Iodogen method

ZHAI Shijun ZHANG Bin YUAN Hang SU Chenghai

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Labeling monoclonal antibody 10D9 with ¹²⁵I using the Iodogen method

更新时间：2021-03-12

- Labeling monoclonal antibody 10D9 with ¹²⁵I using the Iodogen method
- NUCLEAR TECHNIQUES Vol. 32, Issue 5, (2009)
- 作者机构：
  
  苏州大学附属第一医院核医学科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R817
- Published：01 May 2009
- 稿件说明：
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[1]翟士军,章斌,袁航,苏成海.Iodogen法~(125)I标记单克隆抗体10D9[J].核技术,2009,32(05):371-374.

ZHAI Shijun ZHANG Bin YUAN Hang SU Chenghai. Labeling monoclonal antibody 10D9 with ¹²⁵I using the Iodogen method[J]. Nuclear techniques, 2009, 32(5): 371-374.
[1]翟士军,章斌,袁航,苏成海.Iodogen法~(125)I标记单克隆抗体10D9[J].核技术,2009,32(05):371-374. DOI：

ZHAI Shijun ZHANG Bin YUAN Hang SU Chenghai. Labeling monoclonal antibody 10D9 with ¹²⁵I using the Iodogen method[J]. Nuclear techniques, 2009, 32(5): 371-374. DOI：

摘要

采用Iodogen法

选择最佳标记条件进行125I标记单克隆抗体10D9。标记产物用SephadexG-50分离纯化

三氯醋酸（TCA）法测定标记率和放化纯

并对标记物的免疫活性及稳定性进行分析。结果显示

125I标记10D9的最佳条件为Iodogen用量10μg、10D9用量20μg、加入Na125I22.2MBq、室温反应8min

125I-10D9的标记率为（84.10±3.18）%

放化纯为（98.49±1.14）%

比活度为933.51MBq/mg;125I-10D9与Daudi细胞的特异性结合率为（23.21±1.14）%;标记物加到含1%BSA的PBS中放置3W后放化纯度仍>90%。结果表明:Iodogen法125I标记10D9标记率和放化纯高

方法简便

标记物的稳定性好且能较好地保持其免疫活性。

Abstract

To establish the method radiolabeled monoclonal antibody 10D9 against human costimulatory molecules CD80 with 125I

10D9 was labeled with 125I using the Iodogen method at room temperature.The labeling efficiency and radiochemical purity were tested with TCA

and the immune activity and stability of the labeling product were analyzed.The optimum labeling condition was 10 μg Iodogen

20 μg 10D9 and 22.2 MBq Na125I.The reaction time was 8 min at room temperature.The labeled yield was(84.10±3.18)%

and the radiochemical purity was(98.49±1.14)%.The radio-activity of 125I-10D9 was 933.51 MBq/mg.The specific binding efficiency of 125I-10D9 with Daudi cell was(23.21±1.14)%.The radiochemical purity of 125I-10D9 was over 90% after three weeks of mixing with PBS.In conclusion

the method is simple and easy to perform.The labeling efficiency and radiochemical purity is high.The stability of 125I-10D9 is fine in vitro and 125I-10D9 keeps its immune activity after Iodogen labeling.

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Labeling monoclonal antibody 10D9 with 125I using the Iodogen method

DOI：

摘要

Abstract

关键词

Keywords

references

Labeling monoclonal antibody 10D9 with ¹²⁵I using the Iodogen method