<sup>18</sup>F-FHBG PET imaging of HSV1-tk reporter gene expression in tumor xenografts of nude mice

WANG Quanshi LI Hongsheng TANG Ganghua LI Dongli WANG Qiaoyu WU Hubing

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¹⁸F-FHBG PET imaging of HSV1-tk reporter gene expression in tumor xenografts of nude mice

更新时间：2021-03-12

- ¹⁸F-FHBG PET imaging of HSV1-tk reporter gene expression in tumor xenografts of nude mice
- NUCLEAR TECHNIQUES Vol. 32, Issue 5, (2009)
- 作者机构：
  
  南方医科大学南方医院PET中心
- 作者简介：
- 基金信息：
  
  国家自然科学基金(30370426、30870731)资助
- DOI：
  CLC： R73-3
- Published：01 May 2009
- 稿件说明：
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[1]王全师,李洪生,唐刚华,理东丽,王巧愚,吴湖炳.~(18)F-FHBG PET裸鼠移植瘤的HSV1-tk基因显像[J].核技术,2009,32(05):379-382.

WANG Quanshi LI Hongsheng TANG Ganghua LI Dongli WANG Qiaoyu WU Hubing. ¹⁸F-FHBG PET imaging of HSV1-tk reporter gene expression in tumor xenografts of nude mice[J]. Nuclear techniques, 2009, 32(5): 379-382.
[1]王全师,李洪生,唐刚华,理东丽,王巧愚,吴湖炳.~(18)F-FHBG PET裸鼠移植瘤的HSV1-tk基因显像[J].核技术,2009,32(05):379-382. DOI：

WANG Quanshi LI Hongsheng TANG Ganghua LI Dongli WANG Qiaoyu WU Hubing. ¹⁸F-FHBG PET imaging of HSV1-tk reporter gene expression in tumor xenografts of nude mice[J]. Nuclear techniques, 2009, 32(5): 379-382. DOI：

摘要

制备了18F-FHBG基因探针

构建表达HSV1-tk报告基因的逆转录病毒表达载体pLXSN-tk

并建立人肝癌BEL-7402裸鼠双侧腋窝稍靠背侧皮下移植瘤模型。右侧移植瘤为实验组

左侧移植瘤为对照组

将逆转录病毒转染实验组移植瘤细胞。经裸鼠尾静脉注射18F-FHBG后

于10、30、50、70、90、110、130、150、170、190、210min进行PET显像。结果显示:18F-FHBG未校正放化产率为4%-10%

经放射性HPLC分析测定放化纯度>95%。对照组移植瘤放射性分布未见明显增高;实验组移植瘤于注射18F-FHBG后50-210min

PET显像见到灰阶可分辨放射性浓聚

150min前摄取值逐渐升高

150min达到高峰（摄取值为19.772kBq·mL-1）

150min后摄取值快速降低。实验组移植瘤的摄取值明显高于对照组移植瘤

两组移植瘤摄取值比值达4.99。表明18F-FHBGPET报告基因显像系统可用于监测HSV1-tk基因活体内靶细胞内的表达。

Abstract

Radiopharmaceutical of 18F-FHBG was synthesized and recombinant expression plasmid pLXSN-tk was constructed.The nude mice sub-skin model transplanted with human liver cancer was constructed by subcutaneous implantation of cultured the BEL-7402 liver carcinoma cell lines into the two sides axillary fossa nude mice.The left tumors were used as the control

while the right tumors were used as the experiment group

with the cancer cell being transfected by the pLXSN-tk.PET images were taken at 10

110

130

150

170

190 and 210 min after 18F-FHBG injection via the tail veins.The results show that the uncorrected radiochemical yield of 18F-FHBG was 4%-10% and the radiochemical purity was over 95%.The 18F-FHBG PET images indicated that 18F-FHBG uptake of the control did not increase obviously

whereas high activity was observed in the experiment group 50-210 min after 18F-FHBG injection.18F-FHBG uptake of the experiment group increased gradually to 19.772 kBq·mL-1 150 min after 18F-FHBG injection

where the experiment/control tumor ratio was peaked at 4.99.The results indicated that 18F-FHBG PET imaging of HSV1-tk expression in tumors can be used to monitor

in living animals

and expression of theoretical gene.

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Related Author

WU Hubing

ZUO Wei

DONG Ye

ZHOU Wenlan

WANG Quanshi

LI Hongsheng

WU Hubing

Related Institution

Department of PET Center, Nanfang Hospital, Southern Medical University

Department of PET Center,Southern Hospital,Southern Medical University

Institute of Nuclear Science and Technology, Sichuan University

Department of Nuclear Medicine,the Baoan Hospital Affiliated to Nangfang Medical University,Shenzhen 518101,China) 2

广州军区广州总医院核医学科

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18F-FHBG PET imaging of HSV1-tk reporter gene expression in tumor xenografts of nude mice

DOI：

摘要

Abstract

关键词

Keywords

references

¹⁸F-FHBG PET imaging of HSV1-tk reporter gene expression in tumor xenografts of nude mice