Biodistribution analysis of <sup>125</sup>I-albumin-IFN-alpha2b fusion protein in rats

ZHOU Yaoyuan1 ZHANG Rongjun1 CAI Gangming1 GU Xiaobo1 JIANG Mengjun1 ZHANG Bo1 YANG Jianliang2 YANG Min1 CAO Guoxian1 1

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Biodistribution analysis of ¹²⁵I-albumin-IFN-alpha2b fusion protein in rats

更新时间：2021-03-12

- Biodistribution analysis of ¹²⁵I-albumin-IFN-alpha2b fusion protein in rats
- NUCLEAR TECHNIQUES Vol. 32, Issue 2, (2009)
- 作者机构：
  
  1. 江苏省原子医学研究所,卫生部核医学重点实验室
  2. 上海我武生物科技有限公司
- 作者简介：
- 基金信息：
  
  国家自然科学基金(30670589);卫生部科研(wkj2006-2-5)基金;江苏省人事厅“六大人才高峰”计划B类资助(06-B-026);江苏省卫生厅医学重点人才项目(RC2007097)资助
- DOI：
  CLC： R817
- Published：01 February 2009
- 稿件说明：
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[1]周尧远,张荣军,蔡刚明,顾晓波,蒋孟军,张波,杨健良,杨敏,曹国宪.~(125)I-白蛋白融合干扰素α2b大鼠体内分布研究[J].核技术,2009,32(02):150-153.

ZHOU Yaoyuan1 ZHANG Rongjun1 CAI Gangming1 GU Xiaobo1 JIANG Mengjun1 ZHANG Bo1 YANG Jianliang2 YANG Min1 CAO Guoxian1 1. Biodistribution analysis of ¹²⁵I-albumin-IFN-alpha2b fusion protein in rats[J]. Nuclear techniques, 2009, 32(2): 150-153.
[1]周尧远,张荣军,蔡刚明,顾晓波,蒋孟军,张波,杨健良,杨敏,曹国宪.~(125)I-白蛋白融合干扰素α2b大鼠体内分布研究[J].核技术,2009,32(02):150-153. DOI：

ZHOU Yaoyuan1 ZHANG Rongjun1 CAI Gangming1 GU Xiaobo1 JIANG Mengjun1 ZHANG Bo1 YANG Jianliang2 YANG Min1 CAO Guoxian1 1. Biodistribution analysis of ¹²⁵I-albumin-IFN-alpha2b fusion protein in rats[J]. Nuclear techniques, 2009, 32(2): 150-153. DOI：

摘要

采用氯胺-T法对白蛋白融合干扰素α2b进行125I标记

PD-10柱纯化

三氯醋酸沉淀法测定放化纯;体外以WISH/VSV系统比较白蛋白融合干扰素α2b和125I-白蛋白融合干扰素α2b的抗病毒活性;SD大鼠皮下注射125I-白蛋白融合干扰素α2b

分别于给药后0.5、2、6、24、48、90、180、300h放血处死

取血和相关脏器

称重并测定放射性

计算每克脏器放射性占注入量的百分率（%ID?g-1）。结果显示

氯胺-T法制备的125I-白蛋白融合干扰素α2b标记率为82.72%

放化纯95.53%

放射性比活度为0.26MBq/μg;抗病毒活性比较结果表明标记前后的生物学活性几乎无变化;皮下注射大鼠体内分布研究表明

125I-白蛋白融合干扰素α2b在血中于6h达高峰

消除缓慢

未见特异性组织或器官的浓聚。

Abstract

125I-albumin-IFN-alpha2b was prepared with the methods of Ch-T and purified with PD-10 column. The radiochemical purity was measured with TCA (trichloroacetic acid) precipitation. The antiviral activities of 125I-albumin-IFN-alpha2b and albumin-IFN-alpha2b were compared with WISH/VSV system in vitro. SD rats were injected with 125I-albumin-IFN-alpha2b subcutaneously and sacrificed at 0.5

180 and 300 h post-injection. Selected organs were dissected

weighed and their radioactivity was measured using γ-counter. The accumulated radioactivity in the tissues was calculated in terms of percentage of injected dose per gram organ (%ID·g-1). The labeling yield was 82.72%. The radiochemical purity of 125I-albumin-IFN-alpha2b was 95.53%

and its radioactivity was 0.26 MBq/μg. The antiviral bioactivities of albumin-IFN-alpha2b and 125I-albumin- IFN-alpha2b did not change. Biodistribution analysis of 125I-albumin-IFN-alpha2b in rats showed that concentrated 125I- albumin-IFN-alpha2b in blood reached maximum at 6 h post injection

and eliminated slowly. No specific accumulation was seen in other tissues. 125I-albumin-IFN-alpha2b could maintain in peripheral blood for a long time and it meant albumin-IFN-alpha2b would be an effective long-term interferon.

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Biodistribution analysis of 125I-albumin-IFN-alpha2b fusion protein in rats

DOI：

摘要

Abstract

关键词

Keywords

references

Biodistribution analysis of ¹²⁵I-albumin-IFN-alpha2b fusion protein in rats