In this paper

we investigate the radiation effect on the differentiation of bone marrow stromal cells(BMSCs).The local femoral head of rats was irradiated to 30 Gy by 137Cs γ-rays.The BMSCs were cultured two weeks after irradiation.The alkaline phospatase staining

oil red staining and immunohististochemistry were made after the induction

while observing the expression level of Cbf-α1

PPAR-γ

VEGF-a and KDR detected by RT-PCR technology.It was found that the local radiation depressed the differentiation of the BMSCs

with the expression level of Cbf-α1

PPAR-γ

VEGF-a and KDR being all down regulated.Induction could promote the irradiated BMSCs differentiation obviously to osteoblasts

adipocytes and endothelial cells

while the expression of Cbf-α1 and KDR were up-regulated obviously

but the PPAR-γ and VEGF-a were similar to that of the normal BMSCs.These indicate that the radiation depressed the BMSCs to differentiate to osteoblasts

adipocytes and endothelial cells

and after the special induction the differentiation of irradiated BMSCs could recover partly.The expression of correlated genes up-regulated obviously

but the numbers were still fewer for BMSCs differentiated to osteoblasts

adipocytes and endothelial cells.