Induction of APE/Ref-1 expression of human pancreatic cancer cell line Patu-8988 response to colloid of chromic <sup>32</sup>P phosphate

XIONG Guangsu WU Shuming XU Xiaojing ZHOU Jun ZHU Hongyin LI Enling MO Jianzhong

doi:null

您当前的位置：

首页 >

文章列表页 >

Induction of APE/Ref-1 expression of human pancreatic cancer cell line Patu-8988 response to colloid of chromic ³²P phosphate

更新时间：2021-03-12

- Induction of APE/Ref-1 expression of human pancreatic cancer cell line Patu-8988 response to colloid of chromic ³²P phosphate
- NUCLEAR TECHNIQUES Issue 2, (2008)
- 作者机构：
  
  1. 上海交通大学附属仁济医院消化内科上海市消化疾病研究所
  2. ,上海,200001
- 作者简介：
- 基金信息：
  
  上海市领先学科建设项目(Y0205);上海交通大学医学院博士点基金(BXJ0617)资助
- DOI：
  CLC： R735.9
- Published：01 February 2008
- 稿件说明：
移动端阅览
[1]熊光苏,吴叔明,徐晓晶,周鋆,朱红音,李恩灵,莫剑忠.放射性核素~(32)P对人胰腺癌Patu-8988细胞株APE/Ref-1的诱导作用[J].核技术,2008(02):129-132.

XIONG Guangsu WU Shuming XU Xiaojing ZHOU Jun ZHU Hongyin LI Enling MO Jianzhong. Induction of APE/Ref-1 expression of human pancreatic cancer cell line Patu-8988 response to colloid of chromic ³²P phosphate[J]. Nuclear techniques, 2008, (2): 129-132.
[1]熊光苏,吴叔明,徐晓晶,周鋆,朱红音,李恩灵,莫剑忠.放射性核素~(32)P对人胰腺癌Patu-8988细胞株APE/Ref-1的诱导作用[J].核技术,2008(02):129-132. DOI：

XIONG Guangsu WU Shuming XU Xiaojing ZHOU Jun ZHU Hongyin LI Enling MO Jianzhong. Induction of APE/Ref-1 expression of human pancreatic cancer cell line Patu-8988 response to colloid of chromic ³²P phosphate[J]. Nuclear techniques, 2008, (2): 129-132. DOI：

摘要

为研究人胰腺癌细胞株在放射性核素32P胶体内照射时APE/Ref-1基因表达的变化

并试图揭示其在胰腺癌放疗抵抗中所起的作用

用不同剂量的放射性核素32P胶体加入人胰腺癌Patu-8988细胞株培养液中

形成最终放射性活度分别为:0、0.11、0.22、0.45、0.90MBq/mL等5个放射照射剂量组

共孵育24h。然后分别以RT-PCR及Western-blot方法测定作用后APE/Ref-1的mRNA及蛋白表达情况。32P胶体作用24h后发现

Patu-8988的APE/Ref-1的mRNA及蛋白表达水平明显上升

并与32P胶体的放射照射剂量呈正相关。由此得出结论:APE/Ref-1在胰腺癌放射性核素32P胶体内照射时表达明显增强

可能与胰腺癌放疗抵抗的产生有关

并提示针对APE/Ref-1的靶向干预可能有助于提高胰腺癌的放疗敏感性。

Abstract

This work is to investigate expression changes of APE/Ref-1 in human pancreatic cancer cells irradiated with colloid of chromic 32P phosphate (32P-CP). Human pancreatic cancer cell line of Patu-8988 was irradiated with 0 0.11

0.22

0.45 or 0.90 MBq/mL of 32P-CP for 24 h. The mRNA and protein levels of APE/Ref-1 were determined by RT-PCR and Western-blot respective. The results show that the expression of APE/Ref-1

both of mRNA and protein levels were elevated significantly and were positively related to the irradiation dose. These suggested that elevated APE/Ref-1 expression might be an adaptive response contributing to the overall radioresistance seen in pancreatic carcinomas and implied the potential theraputic effects of gene silence targeted to APE/Ref-1.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Cooperation of Ad-hING4 and ¹²⁵Ⅰ seed in tumor-suppression on human pancreatic cancer xenograft in nude mice

Related Author

ZHAI Hongyan1 YANG Jicheng2 SHENG Weihua2 XIE Yufeng2 FA Yihua1 SU Chenghai1 1

杨吉成

盛伟华

谢宇锋

法逸华

苏成海

Related Institution

Department of Nuclear Medicine, No.1 Hospital Affiliated to Suzhou University, Suzhou 215006, China) 2

苏州大学基础医学系细胞与分子生物学教研室

AI问答

Address：2019 Jialuo Road, Jiading, Shanghai, China Postal code：201800
Tel：021-39194977 Email：LHB@sinap.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 沪ICP备05005479号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
Total Visits：881163 Visits Today：690

⁰

Induction of APE/Ref-1 expression of human pancreatic cancer cell line Patu-8988 response to colloid of chromic 32P phosphate

DOI：

摘要

Abstract

关键词

Keywords

references

Induction of APE/Ref-1 expression of human pancreatic cancer cell line Patu-8988 response to colloid of chromic ³²P phosphate