Noninvasive detection of experimental acute venous thrombosis with <sup>99</sup>Tc<sup>m</sup> labeled C2A domain of synaptotagmin I

FANG Wei1 JI Shundong2 WANG Feng3 WANG Feng1 RUAN Changgeng2 WANG Zizheng3 1

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Noninvasive detection of experimental acute venous thrombosis with ⁹⁹Tc^m labeled C2A domain of synaptotagmin I

更新时间：2021-03-12

- Noninvasive detection of experimental acute venous thrombosis with ⁹⁹Tc^m labeled C2A domain of synaptotagmin I
- NUCLEAR TECHNIQUES Issue 2, (2008)
- 作者机构：
  
  1. 中国医学科学院阜外心血管病医院核医学科
  2. 苏州大学附属第一医院江苏省血液研究所
  3. 南京医科大学附属南京第一医院核医学科
  4. ,南京,210006
- 作者简介：
- 基金信息：
  
  国家自然科学基金(30500134)资助
- DOI：
  CLC： R817
- Published：01 February 2008
- 稿件说明：
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[1]方纬,季顺东,王峰,王峰,阮长耿,王自正.~(99)Tc~m标记突触结合蛋白I-C2A片段无创性检测急性静脉血栓[J].核技术,2008(02):133-136.

FANG Wei1 JI Shundong2 WANG Feng3 WANG Feng1 RUAN Changgeng2 WANG Zizheng3 1. Noninvasive detection of experimental acute venous thrombosis with ⁹⁹Tc^m labeled C2A domain of synaptotagmin I[J]. Nuclear techniques, 2008, (2): 133-136.
[1]方纬,季顺东,王峰,王峰,阮长耿,王自正.~(99)Tc~m标记突触结合蛋白I-C2A片段无创性检测急性静脉血栓[J].核技术,2008(02):133-136. DOI：

FANG Wei1 JI Shundong2 WANG Feng3 WANG Feng1 RUAN Changgeng2 WANG Zizheng3 1. Noninvasive detection of experimental acute venous thrombosis with ⁹⁹Tc^m labeled C2A domain of synaptotagmin I[J]. Nuclear techniques, 2008, (2): 133-136. DOI：

摘要

突触结合蛋白I-C2A片段（SytI-C2A）具有与活化血小板膜表面的磷脂酰丝氨酸（PS）特异结合的能力。本研究通过动物实验初步探讨99Tcm标记突触结合蛋白I-C2A片段（99Tcm-SytI-C2A）作为显像剂用于进行急性静脉血栓显像的可行性。选择比格犬5只

通过导管将双股螺旋金属丝送入一侧后肢股静脉

制成急性股静脉血栓模型;然后静脉注射99Tcm-SytI-C2A185MBq

注射后1h、2h和3h分别进行显像

用勾画"感兴趣"区的方法计算血栓部位与对侧后肢的对称部位以及血栓/本底的放射性比值。取出血栓、后肢肌肉和血液等标本

体外测定%ID·g-1值。结果表明

注射后1h、2h和3h

血栓部位与对侧后肢对称部位的放射性比值分别为3.01±0.30、3.22±0.21和3.37±0.57;与同侧下肢放射性本底的比值分别为3.10±0.39、3.32±0.31和3.50±0.45。体外检测结果显示出股静脉血栓的单位质量放射性摄取量是血液的2.40±0.35倍

是后肢肌肉的68.90±45.30倍。99Tcm-SytI-C2A能用于无创性地早期检测静脉血栓

有望成为较理想的新型血栓显像剂。

Abstract

99Tcm labeled C2A domain of synaptotagmin I (99Tcm-Syt I-C2A) is a scintigraphic tracer that binds to phosphatidylserine exposed on activated platelets. This study is to determine the potential of this agent for imaging acute venous thrombosis. A stainless steel was placed in femoral vein for inducing venous thrombi in 5 dogs

which were injected each with 185 MBq of 99Tcm-Syt I-C2A. Images of legs were acquired at 1

2 and 3 h after injection. ROI analysis was applied to measure the thrombus-to-contralateral location and thrombus-to-background ratios. The samples of thrombus

blood

and muscle were separated and were counted in a γ well counter for percent injected dose %ID·g-1. Thrombus-to-blood and thrombus-to-muscle ratios were calculated from the %ID·g-1 value. In in vivo imag- ing

the thrombus-to- contralateral location ratios were 3.01±0.30

3.22±0.21 and 3.37±0.57

respectively

and throm- bus-to-background ratios were 3.10±0.39

3.32±0.31 and 3.50±0.45

respectively

at 1

2 and 3 h after injection. The thrombus-to-blood and thrombus-to-muscle ratios of %ID·g-1 were 2.40±0.35 and 68.90±45.30

respectively. 99Tcm-Syt I- C2A with high venous thrombus uptake is a promising agent for imaging acute venous thrombosis.

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Noninvasive detection of experimental acute venous thrombosis with 99Tcm labeled C2A domain of synaptotagmin I

DOI：

摘要

Abstract

关键词

Keywords

references

Noninvasive detection of experimental acute venous thrombosis with ⁹⁹Tc^m labeled C2A domain of synaptotagmin I