Influence of low-energy N~+ implantation on the transcription activity of retrotransposon WIS2-1A in wheat

ZHAO Huiru~1 GU Yunhong~1 YA Huiyuan~2 JIAO Zhen~1 QIN Guangyong~1 1

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Influence of low-energy N~+ implantation on the transcription activity of retrotransposon WIS2-1A in wheat

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- Influence of low-energy N~+ implantation on the transcription activity of retrotransposon WIS2-1A in wheat
- NUCLEAR TECHNIQUES Issue 10, (2008)
- 作者机构：
  
  1. 河南省离子束生物工程重点实验室郑州大学物理工程学院
  2. 洛阳师范学院生命科学系
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(10505018)资助
- DOI：
  CLC： S512.1
- Published：01 October 2008
- 稿件说明：
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[1]赵慧茹,谷运红,押辉远,焦浈,秦广雍.低能N~+注入对小麦反转录转座子WIS2-1A表达活性的影响[J].核技术,2008(10):761-765.

ZHAO Huiru~1 GU Yunhong~1 YA Huiyuan~2 JIAO Zhen~1 QIN Guangyong~1 1. Influence of low-energy N~+ implantation on the transcription activity of retrotransposon WIS2-1A in wheat[J]. Nuclear techniques, 2008, (10): 761-765.
[1]赵慧茹,谷运红,押辉远,焦浈,秦广雍.低能N~+注入对小麦反转录转座子WIS2-1A表达活性的影响[J].核技术,2008(10):761-765. DOI：

ZHAO Huiru~1 GU Yunhong~1 YA Huiyuan~2 JIAO Zhen~1 QIN Guangyong~1 1. Influence of low-energy N~+ implantation on the transcription activity of retrotransposon WIS2-1A in wheat[J]. Nuclear techniques, 2008, (10): 761-765. DOI：

摘要

本实验采用不同剂量低能N

注入小麦种子

研究种胚在萌动的不同阶段反转录转座子WIS2-1A的表达活动

分析低能N

注入对WIS2-1A表达的时间效应和剂量效应。结果显示种子培养初始的60 h内

室温对照组、真空对照组、0.5×10

/cm

注入组和1.0×10

/cm

注入组的种胚中

反转录转座子WIS2-1A始终维持低水平的表达

且随着种子培养时间延长

表达活性显著下降。经过0.5×10

/cm

处理的小麦

在培养30 h时

种胚内WIS2-1A的相对表达量最高

达到对照的138倍。该发现表明反转录转座子WIS2-1A可能在低能离子束对当代小麦的诱变效应中起一定作用。掌握WIS2-1A对不同剂量低能N

注入的敏感性和它的表达活动规律

能够为低能离子注入生物有机体产生诱变效应的理论研究提供基础。

Abstract

In this work

we studied the transcription activity of retrotransposon WIS2-1A responding to different doses of N

implantation at different growth stages of wheat seeds.The time and dose effect of N

implantation on the transcription of WIS2-1A were analyzed.The results showed that the transcription activity of WIS2-1A decreased with the prolonged growth time in the first 60 h of incubation in the room temperature and vacuum control groups

and the groups implanted with 0.5×10

/cm

and 1.0×10

/cm

.The relative quantification of transcripts in the group implanted with 0.5×10

/cm

and incubated for 30 h was 138 times higher than the control.This indicated that the retrotransposon WIS2-1A probably attributed to the mutation effect of the present generation of wheat.The sensitivity and transcription activity of retrotransposon WIS2-1A responding to N

implantation could ground the theory of mutation effect induced by low-energy ions implantation.

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