Glutaraldehyde fixing and facilitating dispersion of virus particles on mica and HOPG

WANG Peng1; 2 Lü Junhong1 WANG Xinyan1; 2 YANG Haijun1; 2 WANG Huabin1; 2 HUANG Yibo1 LI Bin1 1

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Glutaraldehyde fixing and facilitating dispersion of virus particles on mica and HOPG

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- Glutaraldehyde fixing and facilitating dispersion of virus particles on mica and HOPG
- NUCLEAR TECHNIQUES Issue 7, (2008)
- 作者机构：
  
  中国科学院上海应用物理研究所
- 作者简介：
- 基金信息：
  
  国家重大科学研究计划(2007CB9360003);国家自然科学基金(10674147,10675160);上海市自然科学基金(06ZR14105);中国科学院知识创新工程重要方向项目(KJCX2-YW-H06、KJCX2-YW-M03)资助
- DOI：
  CLC： Q939.4
- Published：01 July 2008
- 稿件说明：
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[1]王鹏,吕军鸿,王鑫艳,杨海军,王化斌,黄一波,李宾.戊二醛交联固定病毒颗粒及其在衬底表面上分散的研究[J].核技术,2008(07):557-560.

WANG Peng1, 2 Lü Junhong1 WANG Xinyan1, 2 YANG Haijun1, et al. Glutaraldehyde fixing and facilitating dispersion of virus particles on mica and HOPG[J]. Nuclear techniques, 2008, (7): 557-560.
[1]王鹏,吕军鸿,王鑫艳,杨海军,王化斌,黄一波,李宾.戊二醛交联固定病毒颗粒及其在衬底表面上分散的研究[J].核技术,2008(07):557-560. DOI：

WANG Peng1, 2 Lü Junhong1 WANG Xinyan1, 2 YANG Haijun1, et al. Glutaraldehyde fixing and facilitating dispersion of virus particles on mica and HOPG[J]. Nuclear techniques, 2008, (7): 557-560. DOI：

摘要

病毒颗粒易于在衬底表面上发生积聚、变形等情况

影响原子力显微镜（atomic force microscope

AFM）对病毒的后续研究。本文采用在重组腺联病毒血清2型（recombinant adeno-associated virus serotype2

rAAV2）病毒溶液中添加戊二醛（glutaraldehyde）的方法

提高病毒在衬底表面上的分散程度。研究结果表明

添加2%（v/v）的戊二醛（glutaraldehyde）不仅可促使病毒颗粒在云母（mica）和高序热解石墨（HOPG）表面上的分散

而且还会减小病毒的形变;随着戊二醛浓度的提高

病毒粒子高度也随之增加

直至接近到20nm。这一方法的建立将有助于AFM对单个病毒颗粒的分析和研究。

Abstract

Atomic force microscope (AFM) is a powerful tool for virus studies. The recombinant adeno-associated virus serotype 2 (rAAV2) has an intendancy to aggregate in liquid or on solid surface. Moreover

changes in virus shape induced by compressing force of the AFM tip hamper studies on virus topography. In this paper

chemical re-agent of glutaraldehyde (2%

v/v) pre-mixed with virus suspension facilitates the dispersion of rAAV2 particles de-posited on both the mica and HOPG surface. In addition

the height of virus particle is up to near 20 nm with the in-crease of concentration of glutaraldehyde.

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