XU Chunyan1, 2 YU Feng1, 2 SUN Lihua1, et al. Structural determination of C-terminal domain of Sau3AI protein by multi-wavelength anomalous dispersion method[J]. Nuclear techniques, 2008, (11): 801-805.
XU Chunyan1, 2 YU Feng1, 2 SUN Lihua1, et al. Structural determination of C-terminal domain of Sau3AI protein by multi-wavelength anomalous dispersion method[J]. Nuclear techniques, 2008, (11): 801-805.DOI:
Structural determination of C-terminal domain of Sau3AI protein by multi-wavelength anomalous dispersion method
The Multi-wavelength Anomalous Dispersion(MAD) method is an important method for three-dimensional structure determination of protein crystals.Diffraction datasets of the C-terminal fragment of Sau3AI crystal were collected at three different wavelengths.The structure of Sau3AI-C was solved by MAD method.It shows that this structure is similar to DNA mismatch repair protein MutH.Structural analysis indicates that the cleft in the middle of the structure is the potential binding site for DNA.