Structural study of human phosphoribosylpyrophosphate synthetase (hPRS1) by SRCD spectroscopy

CHENG Bingbing1 LIU Honglin1 ZHANG Guobin1 LUO Shaofeng2 PENG Xiaohui2 TENG Maikun2 WEI Shiqiang1 1

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Structural study of human phosphoribosylpyrophosphate synthetase (hPRS1) by SRCD spectroscopy

更新时间：2021-03-12

- Structural study of human phosphoribosylpyrophosphate synthetase (hPRS1) by SRCD spectroscopy
- NUCLEAR TECHNIQUES Issue 3, (2008)
- 作者机构：
  
  1. 中国科学技术大学国家同步辐射实验室
  2. 中国科学技术大学合肥微尺度物质科学国家实验室中国科学院结构生物学开放实验室中国科学技术大学生命科学院
- 作者简介：
- 基金信息：
  
  国家自然科学基金重点项目(10635060)资助
- DOI：
  CLC： Q55
- Published：01 March 2008
- 稿件说明：
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[1]程兵兵,刘洪林,张国斌,罗昭锋,彭晓晖,滕脉坤,韦世强.hPRS1蛋白酶的同步辐射真空紫外圆二色光谱研究[J].核技术,2008(03):161-164.

CHENG Bingbing1 LIU Honglin1 ZHANG Guobin1 LUO Shaofeng2 PENG Xiaohui2 TENG Maikun2 WEI Shiqiang1 1. Structural study of human phosphoribosylpyrophosphate synthetase (hPRS1) by SRCD spectroscopy[J]. Nuclear techniques, 2008, (3): 161-164.
[1]程兵兵,刘洪林,张国斌,罗昭锋,彭晓晖,滕脉坤,韦世强.hPRS1蛋白酶的同步辐射真空紫外圆二色光谱研究[J].核技术,2008(03):161-164. DOI：

CHENG Bingbing1 LIU Honglin1 ZHANG Guobin1 LUO Shaofeng2 PENG Xiaohui2 TENG Maikun2 WEI Shiqiang1 1. Structural study of human phosphoribosylpyrophosphate synthetase (hPRS1) by SRCD spectroscopy[J]. Nuclear techniques, 2008, (3): 161-164. DOI：

摘要

人的PRPP合成酶1（hPRS1:Human phosphoribosylpyrophosphate synthetase）是与人体核苷酸代谢途径密切相关的一种重要合成酶。本文利用同步辐射真空紫外圆二色光谱（SRCD）实验方法研究hPRS1二级结构特征

以及通过添加反应底物

观察hPRS1二级结构的变化。研究发现

在添加相应底物的条件下

显示出hPRS1二级结构富有弹性

在外界因素发生改变时

二级结构相应发生较大变化

这个结果表明hPRS1蛋白二级结构的改变对于发挥功能作用是必需的。

Abstract

Human phosphoribosylpyrophosphate synthetase (hPRS1) is an important metabolite essential for nucleo-tide synthesis. The second level structures of hPRS1 before and after binding different reactants were investigated by synchrotron radiation circular dichroism (SRCD) spectroscopy. The results show that the second level structures of hPRS1 after binding reactants possess elasticity and change obviously following the outside factors. The study indi-cates that the transform of second level structures in hPRS1 is necessary for its function.

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