A novel study of abumin human serum radiolabeled with (188)~Re

XIA Jiaoyun1 WANG Yongxian2 YU Junfeng2 YIN Duanzhi2 1

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A novel study of abumin human serum radiolabeled with (188)~Re

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- A novel study of abumin human serum radiolabeled with (188)~Re
- NUCLEAR TECHNIQUES Issue 3, (2008)
- 作者机构：
  
  1. 长沙理工大学化学与环境工程学院
  2. 中国科学院上海应用物理研究所
  3. 放射性药物研究中心
  4. ,上海,201800
- 作者简介：
- 基金信息：
  
  国家自然科学基金(10405034)资助
- DOI：
  CLC： R817
- Published：01 March 2008
- 稿件说明：
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[1]夏姣云,汪勇先,于俊峰,尹端沚.铼[(188)~Re]标记人血清白蛋白的新方法研究[J].核技术,2008(03):214-218.

XIA Jiaoyun1 WANG Yongxian2 YU Junfeng2 YIN Duanzhi2 1. A novel study of abumin human serum radiolabeled with (188)~Re[J]. Nuclear techniques, 2008, (3): 214-218.
[1]夏姣云,汪勇先,于俊峰,尹端沚.铼[(188)~Re]标记人血清白蛋白的新方法研究[J].核技术,2008(03):214-218. DOI：

XIA Jiaoyun1 WANG Yongxian2 YU Junfeng2 YIN Duanzhi2 1. A novel study of abumin human serum radiolabeled with (188)~Re[J]. Nuclear techniques, 2008, (3): 214-218. DOI：

摘要

以含吡啶环的化合物[二（2-吡啶甲基）-氨基]-乙酸（H2L）为双功能螯合剂

使用fac-[188Re（CO）3（H2O）3]+间接标记人血清白蛋白（IgG）

为fac-[188Re（CO）3（H2O）3]+标记蛋白或单抗寻找新方法。Fac-[188Re（CO）3（H2O）3]+标记条件研究表明

以活泼酯188Re（CO）3-L2H-TFP（TFP

6-四氟酚）间接标记IgG时

标记率大于82%。在生理盐水和小牛血清中

48h后产物的放化纯度均大于90%;以fac-[188Re（CO）3（H2O）3]+直接标记IgG作对比

标记率仅60%。在生理盐水和小牛血清中

48h后产物的放化纯度<60%。总的说来

使用fac-[188Re（CO）3（H2O）3]+间接标记生物分子时

具有对称结构的含吡啶环的化合物H2L是潜在的双功能螯合剂。

Abstract

It is a novel method for fac-[188Re(CO)3(H2O)3]+ to radiolabel albumin human serum (IgG) indirectly by the pyridyl-containing bis(2-pyridylmethyl)-amino)-acetic acid (L2H). The results show that radiolabeling efficiency of IgG with 188Re(CO)3-L2H-TFP active ester can be higher than 82% under optimized condition. We studied also the labeling stability over 48 h at 37℃ in 0.9% NaCl or bovine serum albumin with both the labeled products. And it shows that the indirect labeling product (90%) has higher radiochemical purity than the direct labeling product (60%). In summary

the symmetric pyridyl-containing tridentate ligand H2L is a potent bifunctional chelator for the future biomolecules labeling of fac-[188Re(CO)3(H2O)3]+.

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Related Institution

Radiopharmaceutical Center, Shanghai Institute of Applied Physics,the Chinese Academy of Sciences, Shanghai 201800,China ) 2

中国科学院上海应用物理研究所放射性药物研究中心上海201800 长沙理工大学化学与环境工程学院长沙

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University of Chinese Academy of Sciences

Department of Nuclear Medicine, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China) 2

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