Effort of fast-neutron and X-ray on expression of p53 and CyclinB1 of small intestinal tissue in mice

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Effort of fast-neutron and X-ray on expression of p53 and CyclinB1 of small intestinal tissue in mice

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- Effort of fast-neutron and X-ray on expression of p53 and CyclinB1 of small intestinal tissue in mice
- NUCLEAR TECHNIQUES Vol. 31, Issue 8, (2008)
- 作者机构：
  
  1. 苏州大学放射医学与公共卫生学院
  2. 天津大港油田集团职工总医院
- 作者简介：
- 基金信息：
  
  苏州大学医学发展基金重点项目(EE126031)资助;放射医学国家重点学科项目(ZY126601)资助
- DOI：
  CLC： R818
- Published：01 August 2008
- 稿件说明：
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[1]徐冬生,刘芬菊,孙志强,杨学琴,陈秋,周卫芳,陈玲.快中子和X射线照射对小鼠肠P53和CyclinB1蛋白表达的影响[J].核技术,2008,31(08):625-628.

XU Dongsheng1, 2 LIU Fenju1 SUN Zhiqiang1 YANG Xueqin1 CHEN Qiu1 ZHOU Weifang1 CHEN Ling1 1. Effort of fast-neutron and X-ray on expression of p53 and CyclinB1 of small intestinal tissue in mice[J]. Nuclear techniques, 2008, 31(8): 625-628.
[1]徐冬生,刘芬菊,孙志强,杨学琴,陈秋,周卫芳,陈玲.快中子和X射线照射对小鼠肠P53和CyclinB1蛋白表达的影响[J].核技术,2008,31(08):625-628. DOI：

XU Dongsheng1, 2 LIU Fenju1 SUN Zhiqiang1 YANG Xueqin1 CHEN Qiu1 ZHOU Weifang1 CHEN Ling1 1. Effort of fast-neutron and X-ray on expression of p53 and CyclinB1 of small intestinal tissue in mice[J]. Nuclear techniques, 2008, 31(8): 625-628. DOI：

摘要

采用昆明系小鼠

随机分组

快中子和X射线全身照射后6h处死

Western blot法检测小肠组织中p53和CyclinB1蛋白的表达;流式细胞术（Flow cycometry

FCM）检测小肠细胞周期的变化。结果发现

照射后小肠组织p53蛋白的表达量随着照射剂量增加呈上升趋势;CyclinB1蛋白的表达量先是随照射剂量的增加而升高

中子照射组0.28Gy时达到高峰

X射线照射组4Gy时达到高峰

随后逐渐降低;FCM结果显示快中子和X射线照射后

G2/M期细胞数都随照射剂量增高而增多（P<0.05）。以上结果提示低剂量快中子和X射线照射可诱导p53和CyclinB1表达水平的增高

而随着剂量进一步增大可导致CyclinB1表达量的降低

最终发生G2期阻滞。

Abstract

In this paper

effect of fast-neutron and X-rays on the expression of P53 and CyclinB1 in small intestinal tissues of mice were investigated. The mice were randomly grouped and sacrificed 6 h after irradiation. Western blot assay was applied to detect the p53 and CyclinB1 expressions

and flow cytometry was used to monitor changes in the small intestinal cell cycle. The results showed that the expression levels of p53 increased with irradiation dose. The expression levels of CyclinB1 increased with the dose at first

but decreased when the dose exceeded 0.28 Gy. The results from the flow cytometry showed that the amount of cells in G2/M phase increased with the dose in both occasions. It was concluded that the low doses of fast-neutron and X-rays can make the expression levels of p53 and CyclinB1 increase

but the expression levels of CyclinB1 decreased and the G2 arrest occurred with increased doses.

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