Fabrication of high-density protein microarray on an EDTA-PDMS substrate by microcontact printing

YANG Haijun1; 2 LI Hai1 WANG Peng1 LI Bin1 1

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Fabrication of high-density protein microarray on an EDTA-PDMS substrate by microcontact printing

更新时间：2021-03-12

- Fabrication of high-density protein microarray on an EDTA-PDMS substrate by microcontact printing
- NUCLEAR TECHNIQUES Vol. 31, Issue 12, (2008)
- 作者机构：
  
  1. 中国科学院上海应用物理研究所
  2. 中国科学院研究生院
- 作者简介：
- 基金信息：
  
  国家自然科学基金(10674147、10675160);973项目(2007CB936000)资助
- DOI：
  CLC： Q51-3
- Published：01 December 2008
- 稿件说明：
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[1]杨海军,李海,王鹏,李宾.EDTA应用于微接触印刷法制备高密度蛋白微阵列的研究[J].核技术,2008,31(12):946-950.

YANG Haijun1, 2 LI Hai1 WANG Peng1 LI Bin1 1. Fabrication of high-density protein microarray on an EDTA-PDMS substrate by microcontact printing[J]. Nuclear techniques, 2008, 31(12): 946-950.
[1]杨海军,李海,王鹏,李宾.EDTA应用于微接触印刷法制备高密度蛋白微阵列的研究[J].核技术,2008,31(12):946-950. DOI：

YANG Haijun1, 2 LI Hai1 WANG Peng1 LI Bin1 1. Fabrication of high-density protein microarray on an EDTA-PDMS substrate by microcontact printing[J]. Nuclear techniques, 2008, 31(12): 946-950. DOI：

摘要

本文提供了一种在聚二甲基硅氧烷（PDMS）基底上制备高密度蛋白微阵列的新方法。此方法的特点在于通过在PDMS基底上物理吸附乙二胺四乙酸二钠（EDTA）达到提高蛋白质分子转印效率的目的。AFM检测结果表明:转印后的图案结构规整

边缘清晰。测得的蛋白条纹平均宽度约为500nm

条纹间距约为1μm

是一种高密度的蛋白微阵列。

Abstract

In this paper

a new and facile route was developed to efficiently print protein microarrays onto hydrophobic polydimethylsiloxane (PDMS) substrate. The unique feature of this method was that protein could be easily transferred onto PDMS substrate by first physically adsorbing EDTA molecules on the PDMS surface. AFM results showed that the transferred patterns are of high fidelity and density with average width of about 500 nm and the interval of 1μm.

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Statistical length of DNA based on AFM image measured by a computer

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