Cell biology of M-PEIs gene deliver prepared with radiation

DONG Li XU Dongmei HONG Jun GONG Peijun YAO Side

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Cell biology of M-PEIs gene deliver prepared with radiation

更新时间：2021-03-12

- Cell biology of M-PEIs gene deliver prepared with radiation
- NUCLEAR TECHNIQUES Issue 6, (2008)
- 作者机构：
  
  1. 中国科学院上海应用物理研究所
  2. 中国科学院上海应用物理研究所,上海,201800
- 作者简介：
- 基金信息：
  
  国家重点基础研究发展计划973项目(2006CB705700);上海市纳米专项(0243nm027)资助
- DOI：
  CLC： Q78
- Published：01 June 2008
- 稿件说明：
移动端阅览
[1]董黎,徐冬梅,洪军,宫培军,姚思德.辐射制备的M-PEIs基因转导载体细胞生物学研究[J].核技术,2008(06):465-468.

DONG Li XU Dongmei HONG Jun GONG Peijun YAO Side. Cell biology of M-PEIs gene deliver prepared with radiation[J]. Nuclear techniques, 2008, (6): 465-468.
[1]董黎,徐冬梅,洪军,宫培军,姚思德.辐射制备的M-PEIs基因转导载体细胞生物学研究[J].核技术,2008(06):465-468. DOI：

DONG Li XU Dongmei HONG Jun GONG Peijun YAO Side. Cell biology of M-PEIs gene deliver prepared with radiation[J]. Nuclear techniques, 2008, (6): 465-468. DOI：

摘要

将辐射制备的M-PEIs纳米凝胶转导质粒短发夹状RNA（shRNA）

研究了载体复合物的细胞毒性、shRNA转染效率及对靶基因的表达抑制。结果表明M-PEIs纳米凝胶的细胞毒性较低

可以较高基因转染效率将shRNA转移到细胞内

同时携带的质粒shRNA在靶细胞内具有较长的释放期

转染的shRNA能使肝癌细胞的靶基因失活

有效抑制了靶基因的表达

初步证明辐射制备的M-PEIs纳米凝胶是一种有效的基因治疗载体。

Abstract

To study the feasibility of using polyethyleneimine nanoparticles(M-PEIs) prepared by radiation polym-erization as gene deliver

the cytotoxicity of M-PEIs was evaluated by MTS assay.The efficiency of short hairpin RNA(shRNA) transfection and its suppression of the targetted gene expression were investigated by RT-PCR and Western Blotting.It was found that the M-PEIs/shRNA complexes exhibited low cyto-toxicity in HCC.M-PEIs/shRNA also suppressed the target gene expression in liver cancer cell HepG2.The results indicate that M-PEIs have potential as a shRNA delivering agent and can be applied for anti-angiogenic tumor therapy.

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