Mutagenic effects of Brassica napus by <sup>12</sup>C<sup>6+</sup> ion beam

HOU Suiwen1; 2 SUN Landi2 ZHANG Yingcong2 WU Dali2 GUAN Liping2 LI Wenjian1 DANG Bingrong1 XIE Hongmei1 ZHOU Libin1 GAO Qingxiang21

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Mutagenic effects of Brassica napus by ¹²C⁶⁺ ion beam

更新时间：2021-03-12

- Mutagenic effects of Brassica napus by ¹²C⁶⁺ ion beam
- NUCLEAR TECHNIQUES Issue 6, (2008)
- 作者机构：
  
  1. 中国科学院近代物理研究所
  2. 兰州大学生命科学学院干旱与草地生态教育部重点实验室
  3. 兰州730000兰州大学生命科学学院
- 作者简介：
- 基金信息：
  
  中国博士后科学基金(20060390715);“西部之光”联合学者项目(O506160XL0);教育部“新世纪优秀人才支持计划(NCET)”资助
- DOI：
  CLC： S565.4
- Published：01 June 2008
- 稿件说明：
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[1]侯岁稳,孙兰弟,张颖聪,吴大利,管利萍,李文建,党秉荣,颉红梅,周利斌,高清祥.~(12)C~(6+)重离子辐照油菜诱变效应研究[J].核技术,2008(06):449-454.

HOU Suiwen1, 2 SUN Landi2 ZHANG Yingcong2 WU Dali2 GUAN Liping2 LI Wenjian1 DANG Bingrong1 XIE Hongmei1 ZHOU Libin1 GAO Qingxiang21. Mutagenic effects of Brassica napus by ¹²C⁶⁺ ion beam[J]. Nuclear techniques, 2008, (6): 449-454.
[1]侯岁稳,孙兰弟,张颖聪,吴大利,管利萍,李文建,党秉荣,颉红梅,周利斌,高清祥.~(12)C~(6+)重离子辐照油菜诱变效应研究[J].核技术,2008(06):449-454. DOI：

HOU Suiwen1, 2 SUN Landi2 ZHANG Yingcong2 WU Dali2 GUAN Liping2 LI Wenjian1 DANG Bingrong1 XIE Hongmei1 ZHOU Libin1 GAO Qingxiang21. Mutagenic effects of Brassica napus by ¹²C⁶⁺ ion beam[J]. Nuclear techniques, 2008, (6): 449-454. DOI：

摘要

用30Gy、90Gy和180Gy 12C6+重离子辐照油菜干种子

研究其对油菜M1代的诱变效应。结果表明不同剂量12C6+重离子辐照使油菜的出苗率、株高和开花率有不同程度的提高

并使开花期提早;30Gy辐照使单株角果数和单株产量有了一定程度的提高;三种辐照剂量都造成了花粉生活力、千粒重和含油量的降低。RAPD扩增结果表明

42个随机引物中有13个引物扩增出差异条带

30Gy、90Gy和180Gy引起的RAPD变异率分别为22.1%、23.7%和36.2%。研究表明

12C6+重离子辐射能有效地引起油菜DNA序列发生改变

从而诱导基因变异

为油菜育种提供丰富的种质材料。

Abstract

Dry seeds of Brassica napus were irradiated by 12C6+ ion beams with 30

and 180 Gy

and the mutagenic effects of M1 generation were investigated.In comparison to the control group

the irradiated seeds showed positive changes in emerged seedling rate

plant height and blooming rate

with obvious inhibitive effects on pollen vitality

1000-seed weight and seed oil content.The 30 Gy irradiation group outperformed the others in pods per plant and seed yield per plant.By means of RAPD method

the results showed specific bands

such as increasing bands

flawing bands and bands with different fluorescence intensity were observed in 13 primers selected.The variation rates under 30 Gy

90 Gy and 180 Gy 12C6+ irradiation were 22.1%

23.7% and 36.2%

respectively.The research is valuable to the application of 12C6+ heavy ion beam in improving Brassica napus breeding.

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Mutagenic effects of Brassica napus by 12C6+ ion beam

DOI：

摘要

Abstract

关键词

Keywords

references

Mutagenic effects of Brassica napus by ¹²C⁶⁺ ion beam