Screening of an oil-degrading strain by N~+ implantation and the oil degradation conditions

YAN Yajuan1; 2 LI Zongwei1 LIU Jianling2 QIN Guangyong1 1

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Screening of an oil-degrading strain by N~+ implantation and the oil degradation conditions

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- Screening of an oil-degrading strain by N~+ implantation and the oil degradation conditions
- NUCLEAR TECHNIQUES Vol. 31, Issue 12, (2008)
- 作者机构：
  
  1. 郑州大学离子束生物工程省重点实验室
  2. 西北大学生命科学学院
- 作者简介：
- 基金信息：
  
  国家重点基础研究发展规划973项目(2004CB719604);农业部公益性行业科研专项项目(200803034)资助
- DOI：
  CLC： Q933
- Published：01 December 2008
- 稿件说明：
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[1]闫亚娟,李宗伟,刘建玲,秦广雍.低能N~+注入油脂降解菌株的诱变选育及降解条件的初步研究[J].核技术,2008,31(12):915-919.

YAN Yajuan1, 2 LI Zongwei1 LIU Jianling2 QIN Guangyong1 1. Screening of an oil-degrading strain by N~+ implantation and the oil degradation conditions[J]. Nuclear techniques, 2008, 31(12): 915-919.
[1]闫亚娟,李宗伟,刘建玲,秦广雍.低能N~+注入油脂降解菌株的诱变选育及降解条件的初步研究[J].核技术,2008,31(12):915-919. DOI：

YAN Yajuan1, 2 LI Zongwei1 LIU Jianling2 QIN Guangyong1 1. Screening of an oil-degrading strain by N~+ implantation and the oil degradation conditions[J]. Nuclear techniques, 2008, 31(12): 915-919. DOI：

摘要

通过N+注入解脂耶罗威亚酵母（Yarrowia lipolytica）DC-3-2

筛选出一株油脂降解率提高了11.09%的高效菌株DC-3-2-50

经10次传代实验表明该菌株遗传稳定向良好。对DC-3-2-50的降解条件进行了初步研究

结果表明

在最佳条件:初始pH9.0、接种量3.0%、温度为25

8℃、摇床转速为180

00rpm、碳源为大豆油时

其降解率最高

可达87.7%。

Abstract

A strain DC-3-2-50 was obtained through N+ implanting into Yarrowia lipolytica DC-3-2. An increase of 11.09% in the oil-degradation rate was obtained. The stain has good genetic stability after 10 times of subculture. The culturing condition of DC-3-2-50 was studied. The optimal culture conditions were as follow: initial pH value

9.0; inoculum size

3%; temperature

25~28°C; dissolved oxygen

180~200 rpm; and carbon nutriments soybean salad oil. The oil-degradation rate can be up to 87.7%.

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Related Author

WEI Shenglin1

2 WU Lijun2 XIE Chuanxiao2 YU Zengliang2 1

谢传晓

余增亮

YANG Tianyou1 CHEN Linhai1 QIN Guangyong1 LI Zongwei1 SU Mingjie1 WANG Yanping1 CHANG Shenghe1 LI Zongyi2 HUO Yuping1 1

陈林海

秦广雍

李宗伟

Related Institution

Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, the Chinese Academy of Sciences, Hefei 230031) 2

苏州大学城市科学学院苏州

苏州大学城市科学学院

Henan Province Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052) 2

河南师范大学生命科学学院

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