Purification of nanoparticle PCR products and their topography observed with AFM

MI Lijuan 1; 2 LI Bin 1 ZHOU Hualan 1 WANG Hubin1; 2 HU Jun 1 1

doi:null

您当前的位置：

首页 >

文章列表页 >

Purification of nanoparticle PCR products and their topography observed with AFM

更新时间：2021-03-12

- Purification of nanoparticle PCR products and their topography observed with AFM
- NUCLEAR TECHNIQUES Issue 10, (2007)
- 作者机构：
  
  1. 中国科学院上海应用物理研究所
  2. 中国科学院研究生院北京,100049
  3. 中国科学院上海应用物理研究所,上海,201800
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(No.10674147,10675160)资助
- DOI：
  CLC： TB383.1
- Published：01 October 2007
- 稿件说明：
移动端阅览
[1]米丽娟,李宾,周化岚,王化斌,胡钧.纳米粒子PCR产物的纯化及AFM观察[J].核技术,2007(10):876-880.

MI Lijuan 1, 2 LI Bin 1 ZHOU Hualan 1 WANG Hubin1, 2 HU Jun 1 1. Purification of nanoparticle PCR products and their topography observed with AFM[J]. Nuclear techniques, 2007, (10): 876-880.
[1]米丽娟,李宾,周化岚,王化斌,胡钧.纳米粒子PCR产物的纯化及AFM观察[J].核技术,2007(10):876-880. DOI：

MI Lijuan 1, 2 LI Bin 1 ZHOU Hualan 1 WANG Hubin1, 2 HU Jun 1 1. Purification of nanoparticle PCR products and their topography observed with AFM[J]. Nuclear techniques, 2007, (10): 876-880. DOI：

摘要

适量浓度的纳米金可显著改善PCR扩增的特异性

提高扩增的灵敏度和反应速度。本文比较了不同方式处理纳米金优化后扩增产物的纯化效率

同时利用原子力显微镜（AFM）表征了扩增产物的形貌。结果表明

直接纯化纳米粒子PCR产物的纯化效率较高

且纳米金不会对大量扩增产物造成影响

但可能影响少量DNA的形貌;在乙醇沉淀DNA之前的高速离心步骤

虽纯化效率略为降低

少量DNA依然会随纳米金共沉降

但可有效排除扩增产物中的纳米金。

Abstract

Nanoparticle PCR(NP-PCR) is a new method to optimize PCR amplification. Suitable amount of Au nanoparticles can improve specificity

sensitivity and extension rate of PCR. In this paper

we compare efficiency of purifying NP-PCR products with different methods. In addition

topographies of DNA products in NP-PCR were observed with atomic force microscope(AFM) . The results show that most of DNA products purified directly by routing method remain almost free due to less effect of nanoparticales. The yields decrease when the AuNPs were removed by high-speed centrifugation. A little amount of DNA subsided with AuNPs.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Studies on wetting-dewetting process of molecularly thin water adlayer confined between graphene and mica interface

Effect of Fe³⁺ ions on aggregation of glucagon

Related Author

Shuo YANG

Ying WANG

Haijun YANG

Yantao GAO

Jun HU

Jinjin ZHANG

LIU Jingsong LIU Jianhua ZHANG Yingying WU Zhongliang TAN Juhua LIN Dongdong ZHOU Xingfei

刘建华

Related Institution

Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Jiading Campus

University of Chinese Academy of Sciences

Key Laboratory of Nuclear Radiation and Nuclear Energy Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Jiading Campus

Department of Physics,Ningbo University

AI问答

Address：2019 Jialuo Road, Jiading, Shanghai, China Postal code：201800
Tel：021-39194977 Email：LHB@sinap.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 沪ICP备05005479号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
Total Visits：881139 Visits Today：666

⁰