A high sensitive and specific colorimetric mercury(II) biosensor

LIU Xingfen WU Haiping LI Yulan LI Qingnuan WANG Lihua

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A high sensitive and specific colorimetric mercury(II) biosensor

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- A high sensitive and specific colorimetric mercury(II) biosensor
- NUCLEAR TECHNIQUES Issue 5, (2007)
- 作者机构：
  
  1. 中国科学院上海应用物理研究所
  2. 中国科学院上海应用物理研究所,上海,201800
- 作者简介：
- 基金信息：
  
  国家自然科学基金(No.60537030,20404016);上海市纳米专项(No.0452nm068)资助
- DOI：
  CLC： TP212
- Published：01 May 2007
- 稿件说明：
移动端阅览
[1]刘兴奋,武海萍,李玉兰,李晴暖,王丽华.一种高灵敏度、高特异性检测重金属Hg~(2+)的比色生物传感方法[J].核技术,2007(05):467-472.

LIU Xingfen WU Haiping LI Yulan LI Qingnuan WANG Lihua. A high sensitive and specific colorimetric mercury(II) biosensor[J]. Nuclear techniques, 2007, (5): 467-472.
[1]刘兴奋,武海萍,李玉兰,李晴暖,王丽华.一种高灵敏度、高特异性检测重金属Hg~(2+)的比色生物传感方法[J].核技术,2007(05):467-472. DOI：

LIU Xingfen WU Haiping LI Yulan LI Qingnuan WANG Lihua. A high sensitive and specific colorimetric mercury(II) biosensor[J]. Nuclear techniques, 2007, (5): 467-472. DOI：

摘要

巯基修饰的可以与Hg

特异性结合的DNA序列（poly-T）被共价连接在纳米金颗粒表面

构成纳米捕获探针。当检测到Hg2+时

不同纳米金颗粒表面的DNA发生Hg

介导的配对形成准双链结构

纳米金颗粒之间的距离被拉近

导致纳米金颗粒的团聚

颜色由红色变成蓝紫色。检测前

浓度用电感耦合等离子质谱仪（Inductively coupled plasma mass spectrometry

ICP-MS）进行标定

以排除汞盐潮解引起的浓度误差对检测灵敏度的影响。用这种基于纳米金和DNA的生物传感方法检测Hg

检测限最低可达5μmol/L;对Ca

、Mg

等其它8种二价金属离子进行对照实验的结果表明

该方法不仅具有灵敏度高、选择性好的特点

而且方法简单、快速、成本低、便于普及。

Abstract

A high sensitive colorimetric mercury （II） biosensor based on gold nanoparticles （AuNPs） is reported. Thiolated T-rich oligonucleotides were assembled on the surface of AuNPs

which was used as a probe to recognize Hg（II） specifically. The solution was red when the AuNPs were dispersed in the system without target Hg （II）. How- ever

the solution of AuNPs would change to blue when it probed the Hg （II） due to the formation of aggregates of AuNPs. The formation of the T-Hg

-T structure would shorten the distance between the AuNPs

resulting in the for- mation of aggregates and the color change from red to blue that can be observed with naked eyes. This mercury （II） sensor is found to be have high sensitivity and selectivity

as it allows a fast （in minutes） identification of 5μmol/L Hg （II） without any detectable perturbation by a spectrum of non-specific metal ions.

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Related Author

LIU Mengmeng

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CHEN Nan

WANG Lihua

Related Institution

Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Jiading Campus

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