<sup>188</sup>Re labeling of prostate cancer monoclonal antibody and its biodistribution in tumor-bearing nude mice

ZHANG Yong1 CHEN Weizhen1 LU Hanping2 LIANG Changsheng2 LIU Changzheng2 1

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¹⁸⁸Re labeling of prostate cancer monoclonal antibody and its biodistribution in tumor-bearing nude mice

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- ¹⁸⁸Re labeling of prostate cancer monoclonal antibody and its biodistribution in tumor-bearing nude mice
- NUCLEAR TECHNIQUES Issue 8, (2007)
- 作者机构：
  
  1. 中山大学附属第三医院核医学科
  2. 中山大学基础医学院实验核医学教研室
- 作者简介：
- 基金信息：
  
  广东省科技计划项目(2003B30103)
- DOI：
  CLC： R737.25
- Published：01 August 2007
- 稿件说明：
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[1]张勇,陈维真,卢汉平,梁昌盛,刘长征.~(188)Re标记抗人前列腺癌单克隆抗体及其在荷瘤裸鼠体内的放射性分布[J].核技术,2007(08):699-701.

ZHANG Yong1 CHEN Weizhen1 LU Hanping2 LIANG Changsheng2 LIU Changzheng2 1. ¹⁸⁸Re labeling of prostate cancer monoclonal antibody and its biodistribution in tumor-bearing nude mice[J]. Nuclear techniques, 2007, (8): 699-701.
[1]张勇,陈维真,卢汉平,梁昌盛,刘长征.~(188)Re标记抗人前列腺癌单克隆抗体及其在荷瘤裸鼠体内的放射性分布[J].核技术,2007(08):699-701. DOI：

ZHANG Yong1 CHEN Weizhen1 LU Hanping2 LIANG Changsheng2 LIU Changzheng2 1. ¹⁸⁸Re labeling of prostate cancer monoclonal antibody and its biodistribution in tumor-bearing nude mice[J]. Nuclear techniques, 2007, (8): 699-701. DOI：

摘要

为探讨188Re标记抗前列腺癌单克隆抗体7E11C5.3的方法及其生物学分布

采用2-巯基乙醇直接还原法制备188Re-7E11C5.3标记物

常规测定标记率和免疫活性

在荷瘤裸鼠体内检测标记物的生物学分布。结果显示

188Re-7E11C5.3的标记率为（93.16±2.18）%

免疫活性分数为（74.86±1.86）%;经尾静脉注入裸鼠体内后

肿瘤组织摄取率在24h达高峰

非肿瘤组织在4h摄取率较高

以后均随时间延长逐步降低;血液放射性清除迅速;24h时T/NT值达最大

至72h仍可保持在较高水平。结果表明

188Re-7E11C5.3在裸鼠体内对前列腺癌移植瘤有良好的靶向定位作用

可用于前列腺癌放射免疫显像和治疗研究。

Abstract

To investigate radiolabeled monoclonal antibody 7E11C5.3 against human prostate cancer with 188Re and its biodistribution

188Re-7E11C5.3 was prepared by direct 2-mercaptoethanol-reduction method. Labeling efficiency was measured by paper chromatography. Biodistribution of 188Re-7E11C5.3 was studied in nude mice xenografted with human prostate cancer. The labeling yield of 188Re-7E11C5.3 was 93.16±2.18%. The immunoreactive fraction of 188Re-7E11C5.3 was 74.86±1.86%. After 188Re-7E11C5.3 was injected through tail vein of nude mice

the tumor’s uptake peak time was at 24h

while high uptake in non-tumor tissues was observed at 4h. With time elapsed

the uptake gradually decreased. The blood clearance was rapid. The highest T/NT (radio counts of tumor vs non-tumor tissue) was observed at 24h

and high T/NT was kept till 72h. In conclusion

188Re-7E11C5.3 can be located to xenografted human prostate cancer in vivo and can be used for the study of radioimmunoimaging and radioimmunotherapy.

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Related Author

谢瑶

邹俊涛

梁昌盛

ZHANG Yong1 XU Jiehua1 CHEN Weizhen1 LU Hanping2 LIANG Changsheng3 ZOU Juntao4 XIE Yao4 LIU Changzheng2 1

许杰华

陈维真

刘长征

WANG Guohui LIU Changzheng LIANG Changsheng

Related Institution

Department of Nuclear Medicine, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China) 2

中山大学基础医学实验教学中心

中山大学基础医学院人体解剖学教研室

Department of Experimental Nuclear Medicine,Sun Yat-Sen University, Guangzhou 510080) FAN Wei

中山大学肿瘤防治中心核医学科

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188Re labeling of prostate cancer monoclonal antibody and its biodistribution in tumor-bearing nude mice

DOI：

摘要

Abstract

关键词

Keywords

references

¹⁸⁸Re labeling of prostate cancer monoclonal antibody and its biodistribution in tumor-bearing nude mice